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Volume 9, Number 12—December 2003

Dispatch

Noninvasive Method for Monitoring Pneumocystis carinii Pneumonia

Michael J. Linke*, Sandy Rebholz†, Margaret Collins†, Reiko Tanaka†, and Melanie T. Cushion*†Comments to Author 
Author affiliations: *Veterans Affairs Medical Center, Cincinnati, Ohio, USA; †University of Cincinnati College of Medicine, Cincinnati, Ohio, USA

Main Article

Table 1

Efficiencies of the real-time PCR reactionsa

Template Range r2 Slope Amplification Efficiency
mtLSU
0.5 to 0.0005 pgs/rx
0.999
-3.220
2.043
1.043
LH
12.5 to 1.25 x 10-5 ngs/rx
0.966
-3.570
1.906
0.906
OS Undiluted to 1:8 dilution 0.973 -3.328 1.997 0.997

aPCR, polymerase chain reaction; mtLSU, mitochondrial large ribosomal subunit RNA; LH, rat lung homogenate; OS, oral swab P. carinii–specific DNA; rx, reaction.

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