Emerging Infectious Disease ISSN: 1080-6059

Equine Amplification and Virulence of Subtype IE Venezuelan Equine Encephalitis Viruses Isolated during the 1993 and 1996 Mexican Epizootics

Dante Gonzalez*, José G. Estrada-Franco†, Anne-Sophie Carrara†, Judith F. Aronson†, and Scott C. Weaver†

Author affiliations: *Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Mexico City, Mexico; †University of Texas Medical Branch, Galveston, Texas, USA

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Figure 5

Results on 1.5% agarose gel of reverse tranferase–polymerase chain reaction (RT-PCR) products stained with ethidium bromide and imaged under UV light. M: 100-bp marker; H1–4, H10: horses 1–4 and 10; (+): positive control viral RNA, 1.8 x 102 PFU amplified; (N-): negative control for nested PCR (-); negative control from single round RT-PCR. All horse samples and the positive control show a band at the expected size (134 bp), and negative controls show only the primers (below 100 bp).

Figure 5. Results on 1.5% agarose gel of reverse tranferase–polymerase chain reaction (RT-PCR) products stained with ethidium bromide and imaged under UV light. M: 100-bp marker; H1–4, H10: horses 1–4 and 10; (+): positive control viral RNA, 1.8 x 10² PFU amplified; (N-): negative control for nested PCR (-); negative control from single round RT-PCR. All horse samples and the positive control show a band at the expected size (134 bp), and negative controls show only the primers (below 100 bp).

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