Volume 9, Number 3—March 2003
Dispatch
Isolation of Escherichia coli O157:H7 from Intact Colon Fecal Samples of Swine1
Ingrid Feder*
, F. Morgan Wallace*, Jeffrey T. Gray†, Pina Fratamico*, Paula J. Fedorka-Cray†, Rachel A. Pearce‡, Jeffrey E. Call*, Richard Perrine*, and John B. Luchansky*
, F. Morgan Wallace*, Jeffrey T. Gray†, Pina Fratamico*, Paula J. Fedorka-Cray†, Rachel A. Pearce‡, Jeffrey E. Call*, Richard Perrine*, and John B. Luchansky*Table
Characterization of Escherichia coli O157:H7 and non-H7 isolates recovered from 305 swine fecal colon samplesa,b
| Collection date | Swine E. coli O157 isolate no. | E. coli O157 positive colon samples/total colon samples collected | Colon ref. no. | No. of isolates recovered from sample | PCR characteristics | E. coli O157 ribotyping | PFGE type | Antimicrobial resistance patternb | |
|---|---|---|---|---|---|---|---|---|---|
| Feb. 16, 2001 |
1 |
2/5 |
1 |
1 |
rfbO157, fliCH7, stx1, eae, hly933 |
H7 |
1 |
Pan-sensitive |
|
| 2 |
2 |
1 |
rfbO157, fliCH7, stx1, eae, hly933 |
H7 |
1 |
Pan-sensitive |
|||
| Mar. 8, 2001 |
No isolates |
0/20 |
|||||||
| Mar. 22, 2001 |
No isolates |
0/40 |
|||||||
| Apr. 20, 2001 |
3 |
3/40 |
3 |
1 |
rfbO157 |
Non-H7 |
3 |
kan, strept, sulfa, tet |
|
| 4 |
4 |
1 |
rfbO157 |
Non-H7 |
3 |
kan, strept, sulfa, tet |
|||
| 5 |
5 |
1 |
rfbO157 |
Non-H7 |
3 |
kan, sulfa, tet |
|||
| May 4, 2001 |
6–11 |
4/50 |
6 |
6 |
rfbO157, fliCH7, stx1, stx2, eae |
H7 |
2 |
Pan-sensitive |
|
| 12–16 |
7 |
5 |
rfbO157, fliCH7, stx1, stx2, eae |
H7 |
2 |
Pan-sensitive, except isolate no. 15 resistant to strept |
|||
| 17–25 |
8 |
9 |
rfbO157, fliCH7, stx1, stx2, eae |
H7 |
2 |
Pan-sensitive |
|||
| 26,27 |
9 |
2 |
rfbO157, fliCH7, stx1, stx2, eae |
H7 |
2 |
Pan-sensitive |
|||
| May 16, 2001 |
28 |
9/50 |
10 |
1 |
rfbO157 |
Non-H7 |
4 |
Pan-sensitive |
|
| 29 |
11 |
1 |
rfbO157 |
Non- H7 |
4 |
tet |
|||
| 30 |
12 |
1 |
rfbO157 |
Non-H7 |
4 |
tet |
|||
| 31 |
13 |
1 |
rfbO157 |
Non-H7 |
4 |
strept, tet |
|||
| 32 |
14 |
1 |
rfbO157 |
Non- H7 |
4 |
tet |
|||
| 33 |
15 |
1 |
rfbO157 |
Non-H7 |
4 |
tet |
|||
| 34 |
16 |
1 |
rfbO157 |
Non- H7 |
4 |
tet |
|||
| 35 |
17 |
1 |
rfbO157 |
Non- H7 |
4 |
tet |
|||
| 36 |
18 |
1 |
rfbO157 |
Non-H7 |
4 |
tet |
|||
| June 20, 2001 |
No isolates |
0/50 |
|||||||
| July 10, 2001 |
No isolates |
0/50 |
|||||||
| Total=36 | 18/305 | 18 | |||||||
a Each isolate listed in the following table represents an isolate from an individual colon sample.
bFeb., February; Mar., March; Apr., April; ref., reference; PCR, polymerase chain reaction; PFGE, pulsed-field gel electrophoresis; kan, kanamycin; strept, streptomycin; sulfa, sulfamethoxazole; tet, tetracycline.
