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Volume 9, Number 8—August 2003

Research

Community-Acquired Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Genes: Worldwide Emergence

François Vandenesch*, Timothy Naimi†, Mark C. Enright‡, Gerard Lina*, Graeme R. Nimmo§, Helen Heffernan¶, Nadia Liassine#, Michèle Bes*, Timothy Greenland**, Marie-Elisabeth Reverdy*, and Jerome Etienne*Comments to Author 
Author affiliations: *INSERM E0230, Lyon, France; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA; ‡University of Bath, Bath, United Kingdom; §Princess Alexandra Hospital, Brisbane, Australia; ¶Antibiotic Reference Laboratory, 12 Wellington, New Zealand; #Laboratoire Bioanalytique-Riotton, Geneva, Switzerland; **UMR754, Lyon, France

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Figure

Pulsed-field gel electrophoresis (PFGE) pattern and phylogenetic tree of 117 community-acquired (CA)-methicillin resistant Staphylococcus aureus isolates from three continents. SmaI macrorestriction patterns were digitized and analyzed by using Taxotron software (Institut Pasteur, Paris, France) to calculate Dice coefficients of correlation and to generate a dendrogram by the unweighted pair group method using arithmetic averages (UPGMA) clustering. The scale indicates the level of pattern simil

Figure. Pulsed-field gel electrophoresis (PFGE) pattern and phylogenetic tree of 117 community-acquired (CA)-methicillin resistant Staphylococcus aureus isolates from three continents. SmaI macrorestriction patterns were digitized and analyzed by using Taxotron software (Institut Pasteur, Paris, France) to calculate Dice coefficients of correlation and to generate a dendrogram by the unweighted pair group method using arithmetic averages (UPGMA) clustering. The scale indicates the level of pattern similarity. Capital letters indicate macrorestriction types based on visual interpretation of PFGE results. Fragment size (kb) of the reference strain NCTC8325 is indicated on the bottom lane.

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