Volume 10, Number 12—December 2004
Dispatch
Genome Sequence and Attenuating Mutations in West Nile Virus Isolate from Mexico
David W.C. Beasley*
, C. Todd Davis*, Jose Estrada-Franco*, Roberto Navarro-Lopez†, Arturo Campomanes-Cortes†, Robert B. Tesh*, Scott C. Weaver*, and Alan D.T. Barrett*
, C. Todd Davis*, Jose Estrada-Franco*, Roberto Navarro-Lopez†, Arturo Campomanes-Cortes†, Robert B. Tesh*, Scott C. Weaver*, and Alan D.T. Barrett*Table 1
Summary of nucleotide and amino acid differences between West Nile virus strains NY99 (AF196835), NY00-grouse3282 (AF404755), and TM171-03a
| Nucleotide (amino acid) | NY99 | NY00 | TM171-03 |
|---|---|---|---|
| 50 | A | A | G |
| 71 | A | A | G |
| 93 | C | T | C |
| 381 | C | C | T |
| 483 | C | C | T |
| 858 | C | C | T |
| 887 (prM-141) | T (Ile) | T (Ile) | C (Thr) |
| 1137 | C | C | T |
| 1285 | C | T | C |
| 1432 (E-156)b | T (Ser) | T (Ser) | C (Pro) |
| 1626 | C | C | T |
| 2328 | C | C | T |
| 2388 | C | C | T |
| 2466 | C | C | T |
| 2607 | T | T | C |
| 2832 | T | T | C |
| 2865 | C | C | T |
| 3111 | G | G | A |
| 4146 | A | G | G |
| 4212 | T | T | A |
| 4564 | T | C | C |
| 4749 | C | C | T |
| 6120 | C | C | T |
| 6138 | C | T | T |
| 6141 | C | C | T |
| 6279 | G | G | A |
| 6426 | C | T | T |
| 6495 | G | G | A |
| 6771 | A | G | G |
| 7015 | T | C | C |
| 7359 | C | C | T |
| 7648 (NS4B-245) | A (Ile) | A (Ile) | G (Val) |
| 7672 | C | C | T |
| 7938 | T | C | C |
| 8067 | A | G | A |
| 8109 | C | C | T |
| 8676 | A | A | G |
| 8811 | T | C | C |
| 8838 | T | T | C |
| 8994 | T | T | C |
| 9378 | T | T | C |
| 9408 | T | T | C |
| 9453 | C | C | T |
| 10317 | C | C | T |
| 10373 (NS5-898) | C (Thr) | C (Thr) | T (Ile) |
| 10393 | C | T | T |
| 10828 | T | T | G |
| 10851 | A | G | G |
| 10989 | G | G | A |
aBold text indicates residues at which NY00-grouse 3282 and TM171-03 both differed from NY99.
bStrain TM171-03 had a mixed sequence at this residue. Consensus sequence of early passage material had “C” at nucleotide 1432.
