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Volume 10, Number 4—April 2004

Research

Pneumocystis jiroveci Dihydropteroate Synthase Genotypes in Immunocompetent Infants and Immunosuppressed Adults, Amiens, France

Anne Totet*, Sophie Latouche†, Philippe Lacube†, Jean-Claude Pautard*, Vincent Jounieaux*, Christian Raccurt*, Patricia Roux†, and Gilles Nevez*Comments to Author 
Author affiliations: *University Hospital, University of Picardy, Amiens, France; †Saint-Antoine University Hospital, Paris, France

Main Article

Figure 1

Identification of Pneumocystis jiroveci dihydropteroate synthase (DHPS) genotypes. Electrophoresis on 1.5% agarose gel of DHPS polymerase chain reaction products after digestion with AccI (upper line) and HaeIII (lower line). M, molecular weight marker (123 Gibco BRL; Cergy Pontoise, France). Lane 1: double mutant genotype; lanes 2 and 5: wild genotype; lane 3: mixed infection with a wild genotype and a mutant genotype that has the mutation at nucleotide position 165; lane 4: mixed infection wit

Figure 1. Identification of Pneumocystis jiroveci dihydropteroate synthase (DHPS) genotypes. Electrophoresis on 1.5% agarose gel of DHPS polymerase chain reaction products after digestion with AccI (upper line) and HaeIII (lower line). M, molecular weight marker (123 Gibco BRL; Cergy Pontoise, France). Lane 1: double mutant genotype; lanes 2 and 5: wild genotype; lane 3: mixed infection with a wild genotype and a mutant genotype that has the mutation at nucleotide position 165; lane 4: mixed infection with a wild genotype and a mutant genotype which has the mutation at nucleotide position 171.

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