Volume 11, Number 11—November 2005
Dispatch
Vibrio cholerae Pathogenic Clones
Anna Salim*, Ruiting Lan†, and Peter R. Reeves*
Table A1
Chromosomal location of 26 genes of Vibrio cholerae and primers used*
| Gene | Chromosomal location† (kb) | Forward primer | Sequence (5´→3´) | Position‡ | Reverse primer | Sequence (5´→3´) | Position§ | Amplicon size, bp |
|---|---|---|---|---|---|---|---|---|
| gyrB | 11 | 4876 | TGGTTTTTGAGGTGGTGGAT | 116 | 4877 | CGCTTGATTCTTACGGTTAC | 1330 | 1,214 |
| udhA | 141 | 4968 | GAAAAAGAGAGCAGTGTAGG | 106 | 4969 | CGTCCCACTTCATAAGGCAC | 1131 | 1,025 |
| purH | 280 | 4970 | TCAGCGTATCAGATAAAACC | 35 | 4971 | CTTGCCATTCACCACACTCC | 1118 | 1,083 |
| gppA | 314 | 4972 | GCCGTTTCTTCACCCTTGTA | 10 | 4973 | GGATTTTTTGCCAGCCATTC | 1416 | 1,406 |
| lytB | 732 | 4974 | ATGAGCAAACCAATGAAAAT | 1 | 4975 | TTAGGCACTTCAAAGAACAT | 936 | 935 |
| adk | 1050 | 4873 | CATCATTCTTCTCGGTGCTC | 6 | 4967 | CACTCACTTCGCTCACTTGC | 620 | 614 |
| metG | 1102 | 4878 | CTGAACCCCGAAAAAGATAG | 31 | 4879 | CTGTGGAAATAAACGATGTC | 1050 | 1,019 |
| icd | 1209 | 4965 | TTCATTACTGCCGATTATTC | 66 | 4966 | TTTGGTGTCTTTCTGCTTAC | 1129 | 1,063 |
| pepN | 1602 | 5054 | CACACCTCAAGCCAAATACC | 9 | 5055 | GTACAGCTTCATGCCACGTT | 1219 | 1,210 |
| fumC | 1684 | 4874 | TTATCAAGCACAAACTCAAC | 66 | 4875 | TCACAGGCAGCATCACATTA | 1079 | 1,013 |
| metE | 1835 | 4987 | TTGCTCTTGAAAAATACTGG | 59 | 4988 | GAGAAGACTTACGCGCAACA | 1169 | 1,110 |
| ndh | 2038 | 4989 | TTGAGGATTGTTGTTGTGAC | 1 | 4990 | GTTTTAATCATGCCGTGCAG | 1242 | 1,241 |
| plsX | 2177 | 5009 | ATTTAACCGTTGCACTTGAT | 8 | 5010 | GGCTGGGTACTTGTCGTTTC | 980 | 972 |
| purM | 2380 | 4991 | GTGAGCGGTAATAATCCATC | 1 | 4992 | GCTTCGTTGCTATTGGCGGC | 1023 | 1,022 |
| carA | 2557 | 4993 | CAGATGGTATTTCCGTCGGT | 65 | 4994 | AAAGTGGTCAAACAGCGGTG | 1111 | 1,046 |
| pyrG | 2627 | 4995 | GCGGGGTTGTATCCTCTCTA | 29 | 4996 | TATTTGGTGTTTTTATTGAA | 1230 | 1,201 |
| malP¶ | 12 | 5056 | GAAACCTACTCAACAGAAAC | 3 | 5057 | CAGAGTGTGGTTGGTGTAAG | 1090 | 1,087 |
| pncB¶ | 105 | 4999 | GTTTTCTCCCCACATCATTC | 15 | 5000 | TTCACACATGGCTTTTTCCG | 1189 | 1,174 |
| hmpA¶ | 202 | 5001 | TGCTCACCCAAGAACACATC | 2 | 5002 | CGGACCACACAAATAGAA AT | 1081 | 1,079 |
| glyA¶ | 296 | 5003 | ACACCCGCCAAAATGAACAG | 110 | 5004 | CATAGGCTTCTCTTCGTCAA | 1111 | 1,001 |
| fruK¶ | 446 | 5005 | TAACGGGCAGTGTAAACCAG | 47 | 5006 | GCGGATACTTGAATTTGTTG | 930 | 883 |
| pntA¶ | 500 | 5049 | AGAACAACTCGCTGGTGAAA | 21 | 5050 | ATGCCCCTACTACAATGATG | 1409 | 1,388 |
| sdaA¶ | 602 | 4976 | AAGGGGAAAGTTATGCTCTC | 49 | 4977 | CATTTGCTGGTGCGCTTGAG | 1308 | 1,259 |
| speA¶ | 761 | 5058 | TAATCCTCTCTGTTTTTGTG | 47 | 5059 | TGTTTTTCAGTAGCATTGGC | 1214 | 1,167 |
| pyrC¶ | 878 | 5013 | TGACAACACTGACGATTACA | 26 | 5014 | TTGCGAGGTAGGCCGTAGAA | 936 | 910 |
| glgX¶ | 977 | 5060 | GTCAACGCCTTTCCCATTAG | 15 | 5061 | TTCGGCAATCAGCTTTACTT | 1051 | 1,036 |
*The annealing temperatures were 55–62°C for primers for gyrB and pepN and 55°C for the remaining primers.
†Location of each gene on the 2 chromosomes (large and small chromosomes) of V. cholerae isolate N16961.
‡Position of the first base of the forward primer relative to the first base of the start codon.
§Position of the first base of the reverse primer relative to the first base of the start codon.
¶Genes on the small chromosome of V. cholerae isolate N16961.
