Volume 11, Number 8—August 2005
Research
Coxiella burnetii Genotyping
Olga Glazunova*1, Véronique Roux*1, Olga Freylikman*†, Zuzana Sekeyova*‡, Ghislain Fournous*, Judith Tyczka§, Nikolai Tokarevich†, Elena Kovacova‡, Thomas J. Marrie¶, and Didier Raoult*
Table A2
Primer used in djlA, com1 and QpH1 and QpRS plasmid targeted sequences for PCR amplification and sequencing
| Primer | Nucleotide sequence 5´ → 3´ | Position in the gene | |
|---|---|---|---|
| djlA-1*† | CGGTGATGAACTGGATTGG | –5 – 15 | |
| djlA-2† | ATTGACCTGACGCGCTTGACG | 266 –247 | |
| djlA-3† | GGCAACGCAAGACCCCCGTG | 579 – 598 | |
| djlA-4*† | AACCATGCTTCGCACCTTAC | 810 –791 | |
| com-1*† | CGTGAAGAACCGTTTGACTG | 3 – 22 | |
| com-2† | TGAGGATTGCCTGCCACTGG | 284 – 265 | |
| com-3† | GCGCTGCTCAGTGTCGACGG | 490 – 509 | |
| com-4*† | CTTTTCTACCCGGTCGATTTC | 759 – 739 | |
| Primer | Nucleotide sequence 5´ → 3´ | Position in the plasmid | ORF |
| QpH11 | TGACAAATAGAATTTCTTCATTTTGATG | QpH1 (gb:AE016829) 15332 –15359 | Spacer between two hypothetical proteins |
| QpH12 | GCTTATTTTCTTCCTCGAATCTATGAAT | QpH1 (gb:AE016829) 168348 – 16375 | Spacer between two hypothetical proteins |
| QpRSO1 | CTCGTACCCAAAGACTATGAATATATCC | QpRS gb:Y15898) 14761 –14734 | Hypothetical protein |
| QpRS02 | CACATTGGGTATCGTACTGTCCCT | QpRS gb:Y15898) 14398 – 14321 | Hypothetical protein |
| QpDV1f | ATGAGAGAAGAGCAGCCGCT | QpRS (gb:Y15898) 9889 – 9908 | Hypothetical protein |
| QpDV1r | TCAATGATCCGATGTGCGTTT | QpH1 (gb:Y15898) 10634 –10614 | Hypothetical protein |
*Oligonucleotide primer used for PCR amplification.
†Oligonucleotide primer used for sequencing.
1Dr. Glazunova and Dr. Roux contributed equally to this work.
