Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
Asis Khan*, Catherine Jordan*, Cristina Muccioli†, Adriana L. Vallochi‡, Luiz V. Rizzo‡, Rubens Belfort†, Ricardo W.A. Vitor§, Claudio Silveira¶, and L. David Sibley*
Author affiliations: *Washington University School of Medicine, St. Louis, Missouri, USA; †Paulista School of Medicine, São Paulo, Brazil; ‡University of São Paulo, São Paulo, Brazil; §Federal University of Minas Gerais, Belo Horizonte, Brazil; ¶Clinica Silveira, Erechim, Brazil
Figure 2. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analyses of clinical isolates from Brazil compared to analyses of clonal strains. Shown are the PCR markers SAG3 and BTUB, with their respective restriction digests. Alleles are designated below each figure panel and match those given in the Table. Agarose gel electrophoresis of undigested and restriction digested products for type stained (type I RH, type II Me49, type III CTG). Products were resolved on 3% agarose gels strains with ethidium bromide. Mr refers to size markers from φX174 digested with HaeIII.
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