Acanthamoeba Encephalitis in Patient with Systemic Lupus, India
Charudatt G. Shirwadkar*, Rohini Samant*, Milind Sankhe*, Ramesh Deshpande*, Shigeo Yagi†, Frederick L. Schuster†, Rama Sriram‡, and Govinda S. Visvesvara‡
Author affiliations: *P.D. Hinduja National Hospital and Research Centre, Mumbai, India; †California Department of Health Services, Richmond, California, USA; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Figure 2. Results of polymerase chain reaction showing 230-bp and 161-bp bands for Balamuthia (lanes 1–6) and Acanthamoeba (lanes 7–12) mitochondrial 16S rRNA gene DNA. Lane 1, molecular mass marker; lane 2, absence of Balamuthia DNA in patient brain tissue (5-μL sample); lane 3, positive control (Balamuthia DNA); lane 4, negative control (Acanthamoeba DNA); lane 5, negative control (water); lane 6, absence of Balamuthia DNA in patient brain tissue (1-μL sample); lane 7, molecular mass marker; lane 8, Acanthamoeba DNA isolated from patient brain tissue (5-μL sample); lane 9, absence of Balamuthia DNA isolated from amebae in patient sample; lane 10, positive control (Acanthamoeba DNA); lane 11, negative control (water); lane 12, Acanthamoeba DNA isolated from patient brain tissue (1-μL sample).
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.