Volume 13, Number 1—January 2007
Research
Tickborne Relapsing Fever Diagnosis Obscured by Malaria, Togo
Annika Nordstrand*, Ignas Bunikis*, Christer Larsson*, Kodjo Tsogbe†, Tom G. Schwan‡, Mikael Nilsson§, and Sven Bergström*
Table 1
Primers used for cloning, PCR, and DNA sequencing
| Primer | Sequence (5′→3′)* | Reference |
|---|---|---|
| Br_GlpQ_3_BamHI | GGCGGATCCGCTTGACCAGTTGCTCCTCCGC | (16) |
| Br_GlpQ_5_XhoI | GCCGCTCGAGGAAAAGAAAATGCAAAAATAAATAAA | (16) |
| GlpQ For | GGTATGCTTATTGGTCTTC | Present study |
| GlpQ Rev | TTGTATCCTCTTGTAATTG | Present study |
| GlpQ1 | CAAATCACTAAGCCTTAGCGAAAGAT | Present study |
| GlpQ2 | ATCTGTTGGTGCTTCTTCCCAGT | Present study |
| GlpQ3 | CAGGGAAAATTGATAATGCTTGTTGG | Present study |
| GlpQ4 | CTGCTAATGTGAAATCGACGGAATA | Present study |
| Nested_1_F | AGAGTTTGATCCTGGCTTAG | (15) |
| Nested_1_R | CTTGCATATCCGCCTACTCA | (15) |
| Nested_2_F | GGCTTAGAACTAACGCTGGCA | (15) |
| Nested_2_R | CTGCTGGCACGTAATTAGCC | (15) |
*Sequences in boldface indicate restriction sites for BamH1 and XhoI.
