Spread of Cryptococcus gattii in British Columbia, Canada, and Detection in the Pacific Northwest, USA
Laura MacDougall* , Sarah E. Kidd†, Eleni Galanis*, Sunny Mak*, Mira J. Leslie‡, Paul R. Cieslak§, James W. Kronstad†, Muhammad G. Morshed*, and Karen H. Bartlett†
Author affiliations: *British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada; †University of British Columbia, Vancouver, British Columbia, Canada; ‡Washington State Department of Health, Shoreline, Washington, USA; §Oregon State Public Health, Portland, Oregon, USA;
Figure 4. URA5–restriction fragment length polymorphism (RFLP) profiles for selected human, animal, and environmental Cryptococcus gattii isolates. A) URA5-RFLP to determine the molecular type using Hha I and Sau96 I endonucleases (14). B) URA5-RFLP to confirm molecular type and determine VGII subtype, using Hha I, Dde I, and BsrG I endonucleases.
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