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Volume 13, Number 12—December 2007

Letter

Distemper in a Dolphin

Peter Wohlsein*Comments to Author , Christina Puff*, Mihaela Kreutzer*, Ursula Siebert†, and Wolfgang Baumgärtner*
Author affiliations: *University of Veterinary Medicine, Hannover, Germany; †Christian-Albrechts-University, Buesum, Germany;

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Figure

A) Immunohistologic demonstration of morbillivirus antigen in cytoplasm and nuclei of neurons (arrows) and glial cells in the brain of a white-beaked dolphin, using a monoclonal antibody (GenWay, San Diego, CA, USA) against nucleoprotein of canine distemper virus (CDV)/phocine distemper virus (PDV) visible as numerous black dots (magnification ×630). B) Unrooted neighbor-joining phylogenetic tree constructed by using 353 nt from the gene coding for the morbillivirus phosphoprotein. Alignments were calculated with ClustalX version 1.83 (http://bips.u-strasbourg.fr/fr/Documentation/ClustalX). Bootstrapping (values indicated in %) was performed with 1,000 replicates using MEGA 3.1 software (www.megasoftware.net/mega.html). The new isolate from this study is shown in boldface. The following sequences were included: dolphin morbillivirus (DMV) (GenBank accession nos. NC_005283, Z47758, AJ608288), pilot whale morbillivirus (AF200817), Peste-des-petits-ruminants virus (NC_006383), measles virus (NC_001498), Rinderpest virus (NC_006296), CDV (NC_001921), and PDV (D10371). Scale bar shows nucleotide substitutions per site.

Figure. A) Immunohistologic demonstration of morbillivirus antigen in cytoplasm and nuclei of neurons (arrows) and glial cells in the brain of a white-beaked dolphin, using a monoclonal antibody (GenWay, San Diego, CA, USA) against nucleoprotein of canine distemper virus (CDV)/phocine distemper virus (PDV) visible as numerous black dots (magnification ×630). B) Unrooted neighbor-joining phylogenetic tree constructed by using 353 nt from the gene coding for the morbillivirus phosphoprotein. Alignments were calculated with ClustalX version 1.83 (http://bips.u-strasbourg.fr/fr/Documentation/ClustalX). Bootstrapping (values indicated in %) was performed with 1,000 replicates using MEGA 3.1 software (www.megasoftware.net/mega.html). The new isolate from this study is shown in boldface. The following sequences were included: dolphin morbillivirus (DMV) (GenBank accession nos. NC_005283, Z47758, AJ608288), pilot whale morbillivirus (AF200817), Peste-des-petits-ruminants virus (NC_006383), measles virus (NC_001498), Rinderpest virus (NC_006296), CDV (NC_001921), and PDV (D10371). Scale bar shows nucleotide substitutions per site.

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