Figure 3. Western blot of protease-resistant prion protein from TgOvPrP4 mice after proteinase K digestion and immunodetection with anti-PrP Sha31 antibody. A) First passage of typical bovine spongiform encephalopathy (BSE) (lanes 2, 4, and 6) and L-type BSE (lanes 3, 5, and 7). B) First passage of TME in cattle (lanes 2, 4, and 6) and L-type (lanes 3, 5, and 7). C) Second passage of TME in cattle (lanes 2, 4, and 6) and L-type BSE (lanes 3, 5, and 7). Each lane shows PrPres from a distinct individual mouse from each experimental group. Bars to the left of the panel indicate the 29.0- and 20.1-kDa marker positions. Lane 1, PrPres control from a scrapie-infected TgOvPrP4 mouse (C506M3 strain).
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