Figure 2. Phylogenetic analysis of Newcastle disease virus (NDV) vaccine strains unearthed from Istituto Sieroterapico Milanese (ISM), showing the phylogenetic placement of ISM-1 (EU082818), ISM-2 (EU082819), ISM-3 (EU082820), and ISM-4 (EU082818) based on partial F gene necleotide sequences. Sequences determined in this study are in boldface. ISM-1 and ISM-2 belong to La Sota–like cluster; ISM-3 and ISM-4 belong to Herts/33-like cluster. Sequence alignment was achieved with ClustalX version 1.81 (ftp://ftp.ebi.ac.uk/pub/software/unix/clustalx), with sequences from other NDV strains retrieved from GenBank (accession nos. indicated). The phylogenetic study was conducted by using MEGA version 3.1 (www.megasoftware.net). The phylogenetic tree was constructed with the neighbor-joining method.
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