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Volume 13, Number 12—December 2007

Dispatch

Viable Newcastle Disease Vaccine Strains in a Pharmaceutical Dump

Antonella Amendola*Comments to Author , Silvia Bianchi*, Marta Canuti*, Alessandra Zappa*, Giovanna Zanoni†, Raffaella Koncan†, Elisabetta Tanzi*, Giuseppe Cornaglia†, Alessandro Remo Zanetti*, and Giuseppe Tridente†
Author affiliations: *University of Milan, Milan, Italy; †University of Verona, Verona, Italy;

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Figure 2

Phylogenetic analysis of Newcastle disease virus (NDV) vaccine strains unearthed from Istituto Sieroterapico Milanese (ISM), showing the phylogenetic placement of ISM-1 (EU082818), ISM-2 (EU082819), ISM-3 (EU082820), and ISM-4 (EU082818) based on partial F gene necleotide sequences. Sequences determined in this study are in boldface. ISM-1 and ISM-2 belong to La Sota–like cluster; ISM-3 and ISM-4 belong to Herts/33-like cluster. Sequence alignment was achieved with ClustalX version 1.81 (ftp://ftp.ebi.ac.uk/pub/software/unix/clustalx), with sequences from other NDV strains retrieved from GenBank (accession nos. indicated). The phylogenetic study was conducted by using MEGA version 3.1 (www.megasoftware.net). The phylogenetic tree was constructed with the neighbor-joining method.

Figure 2. Phylogenetic analysis of Newcastle disease virus (NDV) vaccine strains unearthed from Istituto Sieroterapico Milanese (ISM), showing the phylogenetic placement of ISM-1 (EU082818), ISM-2 (EU082819), ISM-3 (EU082820), and ISM-4 (EU082818) based on partial F gene necleotide sequences. Sequences determined in this study are in boldface. ISM-1 and ISM-2 belong to La Sota–like cluster; ISM-3 and ISM-4 belong to Herts/33-like cluster. Sequence alignment was achieved with ClustalX version 1.81 (ftp://ftp.ebi.ac.uk/pub/software/unix/clustalx), with sequences from other NDV strains retrieved from GenBank (accession nos. indicated). The phylogenetic study was conducted by using MEGA version 3.1 (www.megasoftware.net). The phylogenetic tree was constructed with the neighbor-joining method.

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