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Volume 13, Number 3—March 2007

Research

In Vitro Cell Culture Infectivity Assay for Human Noroviruses

Timothy M. Straub*Comments to Author , Kerstin Höner zu Bentrup†, Patricia Orosz Coghlan‡, Alice Dohnalkova*, Brooke K. Mayer*1, Rachel A. Bartholomew*, Catherine O. Valdez*, Cynthia J. Bruckner-Lea*, Charles P. Gerba‡, Morteza A. Abbaszadegan§, and Cheryl A. Nickerson†1
Author affiliations: *Pacific Northwest National Laboratory, Richland, Washington, USA; †Tulane University School of Medicine, New Orleans, Louisiana, USA; ‡University of Arizona, Tucson, Arizona, USA; §Arizona State University, Tempe, Arizona, USA;

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Figure 3

Transmission electron microscopy of uninfected and infected cell cultures from the second infection trial. A) Uninfected cells showing normal internal membrane organelles. B) Suspect 29-nm particles in cells, viruses from cell culture lysate from the first infection trial (P1). C) Stool sample flag2 (P0) and D) stool sample 149 (P0) showing numerous 29-nm particles and internal rearrangement of membrane-bound organelles.

Figure 3. Transmission electron microscopy of uninfected and infected cell cultures from the second infection trial. A) Uninfected cells showing normal internal membrane organelles. B) Suspect 29-nm particles in cells, viruses from cell culture lysate from the first infection trial (P1). C) Stool sample flag2 (P0) and D) stool sample 149 (P0) showing numerous 29-nm particles and internal rearrangement of membrane-bound organelles.

Main Article

1Current affiliation: Arizona State University, Tempe, Arizona, USA

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