In Vitro Cell Culture Infectivity Assay for Human Noroviruses
Timothy M. Straub* , Kerstin Höner zu Bentrup†, Patricia Orosz Coghlan‡, Alice Dohnalkova*, Brooke K. Mayer*1, Rachel A. Bartholomew*, Catherine O. Valdez*, Cynthia J. Bruckner-Lea*, Charles P. Gerba‡, Morteza A. Abbaszadegan§, and Cheryl A. Nickerson†1
Author affiliations: *Pacific Northwest National Laboratory, Richland, Washington, USA; †Tulane University School of Medicine, New Orleans, Louisiana, USA; ‡University of Arizona, Tucson, Arizona, USA; §Arizona State University, Tempe, Arizona, USA;
Figure 5. Deconvolved confocal laser scanning micrographs of the molecular beacon fluorescence in situ hybridization assay, demonstrating viral infectivity of a genogroup I virus (Sample 155) and genogroup II virus (flag2). A) Typical response for uninfected cells, no molecular beacon observed. B) Sample 155, P5 in cell culture. C) Sample flag2, P5 in cell culture.
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