Emerging Infectious Disease ISSN: 1080-6059

Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1

Stephen Mark Tompkins*¹

, Zi-Shan Zhao*, Chia-Yun Lo*, Julia A. Misplon*, Teresa Liu*, Zhiping Ye*, Robert J. Hogan†, Zhengqi Wu*, Kimberly A. Benton*, Terrence M. Tumpey‡, and Suzanne L. Epstein*

Author affiliations: *Food and Drug Administration, Bethesda, Maryland, USA; †University of Georgia, Athens, Georgia, USA; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Figure 4

Results of matrix protein 2 (M2) vaccination and booster with DNA prime–adenovirus (Ad), showing cross-reactive antibodies. Mice (8–10 per group) were vaccinated with DNA and given an Ad booster as described in Methods. Immune serum collected 3 weeks after the booster was assayed for immunoglobulin (Ig) G reactive to various M2e peptides by ELISA, as described in Methods. Plates were coated with M2e-PR8 (panel A), M2e-FM (panel B), M2e-H5(SP-83) (panel C), or M2e-H5(HK) (panel D). OD, optical de

Figure 4. Results of matrix protein 2 (M2) vaccination and booster with DNA prime–adenovirus (Ad), showing cross-reactive antibodies. Mice (8–10 per group) were vaccinated with DNA and given an Ad booster as described in Methods. Immune serum collected 3 weeks after the booster was assayed for immunoglobulin (Ig) G reactive to various M2e peptides by ELISA, as described in Methods. Plates were coated with M2e-PR8 (panel A), M2e-FM (panel B), M2e-H5(SP-83) (panel C), or M2e-H5(HK) (panel D). OD, optical density.

Main Article

¹Current affiliation: University of Georgia, Athens, Georgia, USA

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.