Volume 13, Number 7—July 2007
Dispatch
Tickborne Encephalitis, Southwestern France
Bruno Herpe*
, Isabelle Schuffenecker†, Jérome Pillot*, Denis Malvy*, Benjamin Clouzeau*, Nam Bui*, Frederic Vargas*, Didier Gruson*, Hervé Zeller†, Marie E. Lafon*, Hervé Fleury*, and Gilles Hilbert*
, Isabelle Schuffenecker†, Jérome Pillot*, Denis Malvy*, Benjamin Clouzeau*, Nam Bui*, Frederic Vargas*, Didier Gruson*, Hervé Zeller†, Marie E. Lafon*, Hervé Fleury*, and Gilles Hilbert*Table
TBEV diagnosis results*†
| Samples | Days after clinical onset | ELISA TBEV IgM (OD value) | ELISA TBEV IgG (OD value) | PRNT80 TBEV antibody titers | TBEV RT-PCR (10) | Virus culture |
|---|---|---|---|---|---|---|
| CSF | 3 | 1.15 | ND | ND | Negative | ND |
| CSF | 4 | ND | ND | ND | Negative | Negative |
| Serum | 4 | 1.07 | 0.07 | ND | Negative | Negative |
| Serum | 11 | 1.58 | 0.4 | 640 | ND | ND |
| Serum | 24 | 1.65 | 1.82 | 640 | ND | ND |
*TBEV, tickborne encephalitis virus; IgM, immunoglobulin M; OD, optical density; PRNT80: plaque-reduction neutralization test (80%); RT-PCR, reverse transcription–PCR; CSF, cerebrospinal fluid; ND, not done. Boldface type indicates positive values.
†IgM capture and IgG indirect in-house assay were performed to detect IgM and IgG TBEV antibodies. The cut-offs were as follows: IgG cut-off 0.05; IgM cut-off 0.2. A PRNT was performed using the Hypr TBEV strain to determine the titer of TBEV-neutralizing antibodies.
