Virus Detection and Monitoring of Viral Load in Crimean-Congo Hemorrhagic Fever Virus Patients
Roman Wölfel*†, Janusz T. Paweska‡, Nadine Petersen†, Antoinette A. Grobbelaar‡, Patricia A. Leman‡, Roger Hewson§, Marie-Claude Georges-Courbot¶, Anna Papa#, Stephan Günther†, and Christian Drosten†
Author affiliations: *Bundeswehr Institute of Microbiology, Munich, Germany; †Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; ‡National Institute for Communicable Diseases, Sandringham, South Africa; §Health Protection Agency, Porton Down, Salisbury, United Kingdom; ¶Institute Pasteur, Lyon, France; #Aristotle University of Thessaloniki, Thessaloniki, Greece;
Figure 1. Global distribution and phylogenetic relationships of Crimean-Congo hemorrhagic fever virus (CCHFV) strains selected for design and validation of the assay. All strains except those marked with asterisks were tested. Phylogenetic analysis was based on available 450-bp sequences (from the National Center for Biotechnology Information) of CCHFV small (S-) segment and generated by the neighbor-joining method with TreeCon for Windows (version 1.3b; Yves van de Peer, University Konstanz, Germany). Nomenclature of CCHFV clades is based on (7). Note that group VII can be resolved only when analyzing the M-segment, not the s-segment as shown here. *These CCHFV strains are shown for reference, but they were not available for testing. **This strain was not available; however, strain Kosovo, which is almost identical, was tested instead. ***Strain AP92 has also not been available for testing. It was isolated from a Rhipicephalus bursa tick and has never been associated with human disease.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.