Figure 1. A) Anaplasma platys nested PCR products of 30 blood samples from dogs in Chile. Positive samples from dogs 7, 12, 17, 19, 23, and 25 are indicated by a 150-bp band. –, PCR-negative control; dog 13, negative control; M, 50-bp DNA ladder. Value on the right is in basepairs. B) Second-round A. platys groESL nested PCR products of dog DNA samples with 3 sets of primers. Group A, SQ5F/SQ4R (790 bp); group B, SQ3F/SQ4R (1,170 bp); group C, SQ3F/SQ6R (360 bp). M, GeneRuler 1-kb DNA ladder (Fermentas, Hanover, MD, USA); Dog 13, negative control; –, PCR-negative control. Values on the left are in basepairs.