Volume 13, Number 9—September 2007
Dispatch
Anaplasma platys in Dogs, Chile
Katia Abarca*
, Javier López†‡, Cecilia Perret*, Javier Guerrero†, Paula Godoy*, Ana Veloz*, Fernando Valiente-Echeverría*, Ursula León*, Constanza Gutjahr†, and Teresa Azócar*
, Javier López†‡, Cecilia Perret*, Javier Guerrero†, Paula Godoy*, Ana Veloz*, Fernando Valiente-Echeverría*, Ursula León*, Constanza Gutjahr†, and Teresa Azócar*Table 1
Ehrlichia/Anaplasma spp. PCR primers used in this study
| Ehrlichia/Anaplasma spp. (primer type) | Primer | Primer sequence (5′→3′) | Region | Reference |
|---|---|---|---|---|
| Ehrlichia spp., A. phagocytophilum, E. canis, E. chaffeensis, E. ewingii, A. platys (outer) | EHR-OUT1 | CTGGCGGCAAGCCTAACACATGCCAACAT | 16S rRNA | (5) |
| EHR-OUT2 | GCTCGTTGCGGGACTTAACCCAACATCTCACGAC | 16S rRNA | (5) | |
| Ehrlichia spp. (inner) | GE2F | GTTAGTGGCATACGGGTGAAT | 16S rRNA | (5) |
| EHRL3-IP2 | TCATCTAATAGCGATAAATC | 16S rRNA | (5) | |
| A. phagocytophilum (inner) | ge9f | AACGGATTATTCTTTATAGCTTGCT | 16S rRNA | (6) |
| ge2 | GGCAGTATTAAAAGCAGCTCCAGG | 16S rRNA | (6) | |
| E. canis, E. chaffeensis, E. ewingii (inner) | HE3-R | CTTCTATAGGTACCGTCATTATCTTCCCTAT | 16S rRNA | (5) |
| E. canis (inner) | E. canis | CAATTATTTATAGCCTCTGGCTATAGGAA | 16S rRNA | (5) |
| E. chaffeensis (inner) | E. chaffeensis | CAATTGCTTATAACCTTTTGGTTATAAATA | 16S rRNA | (5) |
| E. ewingii (inner) | E. ewingii | CAATTCCTAAATAGTCTCTGACTATT | 16S rRNA | (5) |
| E. equi (inner) | E. equi-3-IP2 | GTCGAACGGATTATTCTTTATAGCTTG | 16S rRNA | (5) |
| E. platys (inner) | EHRL3-IP2 | TCATCTAATAGCGATAAATC | 16S rRNA | (5,7) |
| E. platys | GATTTTTGTCGTAGCTTGCTA | 16S rRNA | (7) | |
| E. platys (outer) | EEgro1F | GAGTTCGACGGTAAGAAGTTCA | groESL | (8) |
| EEgro2R | CAGCGTCGTTCTTACTAGGAAC | groESL | (8) | |
| A. platys (inner) | SQ3F | ATTAGCAAGCCTTATGGGTC | groESL | (9) |
| SQ5F | TCAGTGTGTGAAGGAAGTTG | groESL | (9) | |
| SQ4R | CTTTAGGCTATCAAGAGATG | groESL | (9) | |
| SQ6R | TGCTTCCTATGTTCTTATCG | groESL | (9) |
