Emerging Infectious Disease ISSN: 1080-6059

Human Illnesses Caused by Opisthorchis felineus Flukes, Italy

Orlando Armignacco, Luciano Caterini, Gianluca Marucci, Fabrizio Ferri, Giuliana Bernardini, Giampaolo Natalini Raponi, Alessandra Ludovisi, Teresa Bossù, Maria A. Gomez Morales, and Edoardo Pozio

Author affiliations: Belcolle Hospital, Viterbo, Italy (O. Armignacco, L. Caterini, F. Ferri, G. Bernardini); Istituto Superiore di Sanità, Rome, Italy (G. Marucci, A. Ludovisi, M.A. Gomez Morales, E. Pozio); S. Camillo de Lellis Hospital, Rieti, Italy (G. Natalini Raponi); Istituto Zooprofilattico Sperimentale of Latium and Tuscany, Rome (T. Bossù)

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Figure 2

Alignment of the internal transcribed spacer 2 region of rDNA. Patient 1, DNA from eggs of a patient of the August 2007 outbreak; patient 2, DNA from eggs of the patient of the October–November 2007 outbreak; metacercaria collected from a tench (Tinca tinca) from Lake Bolsena (Latium region, central Italy); Opisthorchis felineus, reference DNA from an adult worm of O. felineus from Germany; O. viverrini, reference DNA from an adult worm of O. viverrini from Thailand; C. sinensis, DNA from an adult worm of Clonorchis sinensis from China. Primer sequences are underlined, different bases are in boldface, and gaps are represented by dashes.

Figure 2. Alignment of the internal transcribed spacer 2 region of rDNA. Patient 1, DNA from eggs of a patient of the August 2007 outbreak; patient 2, DNA from eggs of the patient of the October–November 2007 outbreak; metacercaria collected from a tench (Tinca tinca) from Lake Bolsena (Latium region, central Italy); Opisthorchis felineus, reference DNA from an adult worm of O. felineus from Germany; O. viverrini, reference DNA from an adult worm of O. viverrini from Thailand; C. sinensis, DNA from an adult worm of Clonorchis sinensis from China. Primer sequences are underlined, different bases are in boldface, and gaps are represented by dashes.

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