Volume 14, Number 12—December 2008
Dispatch
Novel Borna Virus in Psittacine Birds with Proventricular Dilatation Disease
Kirsi S. Honkavuori, H.L. Shivaprasad, Brent L. Williams, Phenix-Lan Quan, Mady Hornig, Craig Street, Gustavo Palacios, Stephen K. Hutchison, Monique Franca, Michael Egholm, Thomas Briese
, and W. Ian Lipkin
, and W. Ian LipkinTable 1
Real-time PCR measurement of viral sequences in birds with PPD*
| Case no. | Species† | Age/sex | PDD/ control | Primer/probe set | Organ | Ct | Virus copies‡ (300 ng total RNA) |
|---|---|---|---|---|---|---|---|
| 1034 | Canindae macaw | 30 y/M | PDD | 1034–1322 | Brain | 17.94 | 8.88 × 107 |
| Adrenal gland | 20.51 | 8.77 × 106 | |||||
| Proventriculus and gizzard |
25.26 |
1.22 × 105 |
|||||
| 1322 | Vinaceous Amazon | 1 y/M | PDD | 1034–1322 | Brain | 17.64 | 1.17 × 108 |
| Proventriculus |
28.47 |
6.76 × 103 |
|||||
| 1367 | Canindae macaw | 30 y/F | PDD | 1367 | Brain | 27.33 | 8.89 × 103 |
| Adrenal gland | 18.92 | 3.87 × 106 | |||||
| Proventriculus |
23.82 |
1.13 × 105 |
|||||
| 5473 |
Leadbetter’s cockatoo |
17 y/M |
Control |
1034–1322 and 1367 |
Brain |
>36§ |
Negative |
| 4858 |
Long-tailed parakeet |
Adult/M |
Control |
1034–1322 and 1367 |
Brain |
>36§ |
Negative |
| 2020 |
Cockatiel |
Young/M |
Control |
1034–1322 and 1367 |
Brain |
>36§ |
Negative |
| 3616 | Eclectus parrot | 0.25 y/F | Control | 1034–1322 and 1367 | Brain | >36§ | Negative |
*PPD, poventricular dilatation disease; Ct, cycle threshold.
†All birds were captive animals from California, USA.
‡Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
§A Ct of >36 was rated as negative based on the highest dilution of standard representing 5 copies.
