Volume 14, Number 3—March 2008
Research
Genetic Variability of West Nile Virus in US Blood Donors, 2002–2005
Andriyan Grinev*, Sylvester Daniel*, Susan Stramer†, Susan Rossmann‡, Sally Caglioti§, and Maria Rios*
Table 2
Primer sets used for PCR analyses of West Nile virus and sizes of overlapping amplicons*
| Set/location | Amplicon size, kb | Name | Sequence (5′ → 3′) |
|---|---|---|---|
| 1/F1–R1300 | 1.3 | F1 | AGTAGTTCGCCTGTGTGAGCTGAC |
| R1300 | TTGGCGCATGTGTCAATGCT | ||
| 2/F980–R2000 | 1.0 | F980 | CTTGGAATGAGCAACAGAGA |
| R2000 | GTTAGGTCGTTCAATGAAGC | ||
| 3/F1690–R2685 | 1.0 | F1690 | GAGACGTTAATGGAGTTTGA |
| R2670 | CTTCACTGCTTCCCACATTTG | ||
| 4/F2340–R3420 | 1.1 | F2340 | TTCGGAGGCATGTCCTGGAT |
| R3420 | CTGATCTCCATACCATACCAACA | ||
| 5/F3330–R4120 | 0.8 | F3330 | GAGAGCTGCGGACACCGTGGACC |
| R4120 | CATAGCAGACTTGCTCCTTTCT | ||
| 6/F4070–R4950 | 0.9 | F4070 | CTGTTGATGGTCGGAATAGG |
| R4950 | CCTGGTTTCGTCTGGACGTT | ||
| 7/F4810–R5650 | 0.85 | F4810 | CGCCTGGACCCATACTGG |
| R5650 | CCATTCGTATCCAGAGTTCCA | ||
| 8/F5510–R6430 | 0.9 | F5510 | AGCATTGCAGCAAGAGGTTA |
| R6430 | TAGTGCCTGGTGATCCGAGTACAC | ||
| 9/F6290–R6770 | 0.5 | F6290 | CGACCGGAGGTGGTGCTTTGATGG |
| R6770 | CCTGGAACTTCAGCCATCCA | ||
| 10/F6690–R7550 | 0.85 | F6690 | CCTCCTCATGCAGCGGAA |
| R7550 | GAGCTTGCTCCATTCTCCCA | ||
| 11/F7420–R8260 | 0.85 | F7420 | CCACACCCATCATGCAGAA |
| R8260 | CGTTGGAGCAGCTCCATCTT | ||
| 12/F8170–R9050 | 0.9 | F8170 | CATAGGACGATTCGGGTCCT |
| R9050 | CTCTTTCCCATCATGTTGTAAATGC | ||
| 13/F8920–R9810 | 0.9 | F8920 | CAGCTTTGGGTGCCATGTT |
| R9810 | GAACCTGCTGCCAATCATACC | ||
| 14/F9750–R10630 | 0.9 | F9750 | TCCTCAATGCTATGTCAAAGGT |
| R10630 | GGTCCTCCTTCCGAGACGGT | ||
| 15/F10550–R11029 | 0.5 | F10550 | TGAGTAGACGGTGCTGCCTG |
| R11029 | GATCCTGTGTTCTCGCACCACCAG |
*Internal sequencing primers were also used and their sequences are available upon request.
