Emerging Infectious Disease ISSN: 1080-6059

Discovering and Differentiating New and Emerging Clonal Populations of Chlamydia trachomatis with a Novel Shotgun Cell Culture Harvest Assay

Naraporn Somboonna*†, Sally Mead*, Jessica Liu†, and Deborah Dean*†‡

Author affiliations: *Children’s Hospital Oakland Research Institute, Oakland, California, USA; †University of California, Berkeley, California, USA; ‡University of California School of Medicine, San Francisco, California, USA;

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Figure 1

Photographs and optical microscopy views of the wells showing plaques formed by Chlamydia trachomatis F/IC-Cal3 (A, C, E) and no plaque formed by clinical F persistent strain (B, D, F). A) Single well showing 2 distinct plaques (indicated by arrows). B) Well showing no plaque morphology. C) and D) Optical microscopy image showing plaque areas with little or no neutral red staining (arrows) surrounded by viable cells stained red (magnification ×100). Higher magnification (×400) showed numerous cells that had been infected by reference strain F/IC-Cal3 (E) and the clinical persistent F strain (F).

Figure 1. Photographs and optical microscopy views of the wells showing plaques formed by Chlamydia trachomatis F/IC-Cal3 (A, C, E) and no plaque formed by clinical F persistent strain (B, D, F). A) Single well showing 2 distinct plaques (indicated by arrows). B) Well showing no plaque morphology. C) and D) Optical microscopy image showing plaque areas with little or no neutral red staining (arrows) surrounded by viable cells stained red (magnification ×100). Higher magnification (×400) showed numerous cells that had been infected by reference strain F/IC-Cal3 (E) and the clinical persistent F strain (F).

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