Discovering and Differentiating New and Emerging Clonal Populations of Chlamydia trachomatis with a Novel Shotgun Cell Culture Harvest Assay
Naraporn Somboonna*†, Sally Mead*, Jessica Liu†, and Deborah Dean*†‡
Author affiliations: *Children’s Hospital Oakland Research Institute, Oakland, California, USA; †University of California, Berkeley, California, USA; ‡University of California School of Medicine, San Francisco, California, USA;
Figure 2. Diagram of the cell culture and shotgun harvest assay for Chlamydia trachomatis clinical strains propagated in McCoy cells. Serial dilutions of each clinical sample were used for inoculating wells 1 to 6. IAO, initial agarose overlay; FAO, final agarose overlay.
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