Discovering and Differentiating New and Emerging Clonal Populations of Chlamydia trachomatis with a Novel Shotgun Cell Culture Harvest Assay
Naraporn Somboonna*†, Sally Mead*, Jessica Liu†, and Deborah Dean*†‡
Author affiliations: *Children’s Hospital Oakland Research Institute, Oakland, California, USA; †University of California, Berkeley, California, USA; ‡University of California School of Medicine, San Francisco, California, USA;
Figure 4. Neighbor-joining trees representing evolutionary relatedness of the 19 reference strains, Chlamydophila abortus and 5 clonal variants based on ompA nucleotide (A) and amino acid (B) sequence alignments. The trees were constructed from ClustalW 1.8 alignment (www.ebi.ac.uk/Tools/clustalw2/index.html), and the values at the nodes are the bootstrap confidence levels calculated from 1,000 bootstrap resamplings. See Materials and Methods for details.
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