Emerging Infectious Disease ISSN: 1080-6059

Serologic Evidence for Novel Poxvirus in Endangered Red Colobus Monkeys, Western Uganda

Tony L. Goldberg*

, Colin A. Chapman†‡, Kenneth Cameron‡, Tania Saj†, William B. Karesh‡, Nathan D. Wolfe§, Scott W. Wong¶, Melissa E. Dubois¶, and Mark K. Slifka¶

Author affiliations: *University of Illinois, Urbana, Illinois, USA; †McGill University, Montreal, Quebec, Canada; ‡Wildlife Conservation Society, Bronx, New York, USA; §University of California, Los Angeles, California, USA; ¶Oregon Health and Science University, Portland, Oregon, USA;

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Appendix Figure

Detection of Orthopoxvirus (OPV)-reactive antibodies in red colobus. Vaccinia virus (VV)-coated ELISA plates were used to test for antipoxvirus antibodies by endpoint dilution analysis as previously described (7). As positive controls, a representative VV-immune human (VV+ human) at 1 y postvaccination with DryVax (Wyeth Pharmaceuticals, Madison, NJ) and a modified vaccinia Ankara (MVA)-immune rhesus macaque (MVA+ RM) at 2 months post-vaccination with MVA are included for comparison. Negative controls included 2 unvaccinated human participants (OPV- human) and 6 unvaccinated rhesus macaques (OPV- RM). The dashed line indicates the cut-off value for a seropositive antibody response (200 ELISA units).

Appendix Figure. Detection of Orthopoxvirus (OPV)-reactive antibodies in red colobus. Vaccinia virus (VV)-coated ELISA plates were used to test for antipoxvirus antibodies by endpoint dilution analysis as previously described (7). As positive controls, a representative VV-immune human (VV+ human) at 1 y postvaccination with DryVax (Wyeth Pharmaceuticals, Madison, NJ) and a modified vaccinia Ankara (MVA)-immune rhesus macaque (MVA+ RM) at 2 months post-vaccination with MVA are included for comparison. Negative controls included 2 unvaccinated human participants (OPV- human) and 6 unvaccinated rhesus macaques (OPV- RM). The dashed line indicates the cut-off value for a seropositive antibody response (200 ELISA units).

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