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Volume 14, Number 8—August 2008

Dispatch

Genotyping Rickettsia prowazekii Isolates

Yong Zhu*, Aaron Medina-Sanchez*, Donald Bouyer*, David H. Walker*, and Xue-jie Yu*Comments to Author 
Author affiliations: *University of Texas Medical Branch, Galveston, Texas, USA;

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Table 2

Genotypes of Rickettsia prowazekii strains determined by nucleotide mutation in multiple loci

Strain rp028*
rp181
rp195
rp272–rp273
rp308–rp309
rp691–rp692
GT
268† 286 480 732 713–714 140 1529 52–53 306 1306–1307 1415
GvV-250 T G C TACTTCAAGCTCATTTCG C AA GTCATTATCGTAT TT G 1
GvF-16 T G T TACTTCAAGCTCATTTCG C AA GTCATTATCGTAT TT G 2
Breinl T A C TACTTCAAGCTCATTTCG G GTCATTATCGTAT 3
Cairo T A C G TACTTCAAGCTCATTTCG G A GTCATTATCGTAT 4
ZRS G A C G G AA TT 5
Addis Ababa G A C G G AA TT 5
Madrid E G A C A GG G AA TT 6
Evir G A C GG G AA TT 7

*Gene names or intergenic spacers between genes.
†Positions of nucleotides with mutation, which were counted from the first nucleotide of the coding sequence or the first nucleotide after the stop codon in the case of intergenic spacers; –, deletion of nucleotides, in which the number of nucleotides deleted equals the nucleotides in the same column for the corresponding strains that do not have the deletion. For example, in rp181, the GvV-250 strain has 1 deleted nucleotide when compared with the Cairo strain, but it has deleted 2 nucleotides when compared with the E strain.

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