Volume 15, Number 2—February 2009
Dispatch
Enteroviruses in Patients with Acute Encephalitis, Uttar Pradesh, India
Gajanan N. Sapkal, Vijay P. Bondre, Pradip V. Fulmali, Pooja Patil, Vipul Dadhania, Vijay M. Ayachit, Daya Gangale, K.P. Kushwaha, A.K. Rathi, Shobha D. Chitambar, Akhilesh Chandra Mishra, Milind M. Gore
, and Gopalkrishna
, and GopalkrishnaTable 1
Primers used for PCR and sequencing for enterovirus isolates from encephalitis patients, Uttar Pradesh, India, 2006*
| Serial no. | Region | Location | Primer sequence (5′ → 3′) | Product size, bp |
|---|---|---|---|---|
| 1 | 5’ NCR | 64–84 | CGGTACCTTTGTACGCCTGT | 537 |
| 2 | 5’ NCR | 601–582 | ATTGTCACCATAAGCAGCCA | 537 |
| 3 | 5’ NCR | 166–186 | CAAGCACTTCTGTTTCCCCGG | 400 |
| 4 | 5’ NCR | 566–546 | GAAACACGGACACCCAAAGTA | 400 |
| 5 | VP1/2A | EV-012 (2917–2936) | ATGTAYGTICCICCIGGIGG | 457 |
| 6 | VP1/2A | EV-040 (2917–2936) | ATGTAYRTICCIMCIGGIGC | 457 |
| 7 | VP1/2A | EV-011 (3374–3355) | GCICCIGAYTGITGICCRAA | 457 |
| 8 | VP1 cDNA | AN32 (3009–3002) | GTYTGCCA | NA |
| 9 | VP1 cDNA | AN33 (3009–3002) | GAYTGCCA | NA |
| 10 | VP1 cDNA | AN34 (3111–3104) | CCRTCRTA | NA |
| 11 | VP1 cDNA | AN35 (3009–3002) | RCTYTGCCA | NA |
| 12 | VP3 | 224(1977–1996) | GCIATGYTIGGIACICAYRT | 762 |
| 13 | VP1 | 222 (2969–2951) | CICCIGGIGGIAYRWACAT | 762 |
| 14 | VP1 | AN89 (2602–2627) | CCAGCACTGACAGCAGYNGARAYNGG | 348–393 |
| 15 | VP1 | AN88 (2977–2951) | TACTGGACCACCTGGNGGNAYRWACAT | 348–393 |
*NCR, noncoding region of viral genome; VP, virion protein; NA, not applicable.
