Emerging Infectious Disease ISSN: 1080-6059

Bacterial Phenotype Variants in Group B Streptococcal Toxic Shock Syndrome1

Parham Sendi²

, Linda Johansson, Samira Dahesh, Nina M. Van Sorge, Jessica Darenberg, Mari Norgren, Jan Sjölin, Victor Nizet, and Anna Norrby-Teglund

Author affiliations: Karolinska Institutet, Stockholm, Sweden (P. Sendi, L. Johansson, A. Norrby-Teglund); Basel University Medical Clinic, Liestal, Switzerland (P. Sendi); University of California San Diego, La Jolla, California, USA (S. Dahesh, N.M. Van Sorge, V. Nizet); Swedish Institute for Infectious Disease Control, Stockholm (J. Darenberg); Umeå University, Umeå, Sweden (M. Norgren); Uppsala University, Uppsala, Sweden (J. Sjölin)

Main Article

Figure 3

A) Difference in pigmentation of the low hemolytic (LH)/high encapsulation (HC) and NEM316 wild-type (WT) strains and their corresponding transformants expressing the covR/S locus of the high hemolytic (HH)/low encapsulation (LC) variant after overnight culture. B) CAMP testing with strains displayed according to panel A. The LH/HC variant and the NEM316 strain display a stronger reaction (arrows) than their corresponding transformants. C) Semiquantitative analysis of mRNA expression of CAMP fac

Figure 3. A) Difference in pigmentation of the low hemolytic (LH)/high encapsulation (HC) and NEM316 wild-type (WT) strains and their corresponding transformants expressing the covR/S locus of the high hemolytic (HH)/low encapsulation (LC) variant after overnight culture. B) CAMP testing with strains displayed according to panel A. The LH/HC variant and the NEM316 strain display a stronger reaction (arrows) than their corresponding transformants. C) Semiquantitative analysis of mRNA expression of CAMP factor (cftb), β-h/c (cylK and cylE) capsule (cpsG), and gyrA (housekeeping gene) using reverse transcription–PCR. Lane 1, LH/HC; lane 2, HH/LC; lane 3, LH/HC + pcovR HH; lane 4, NEM316 WT; lane 5, NEM316 + pcovR HH.

Main Article

¹Part of this work was presented at Lancefield 2008 International Symposium on Streptococci and Streptococcal Diseases, Porto Heli, Greece, June 22–26, 2008.

²Current affiliation: Clinic for Infectious Diseases, University Hospital Bern, Bern, Switzerland.

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.