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Volume 15, Number 3—March 2009

Dispatch

Detection of Newly Described Astrovirus MLB1 in Stool Samples from Children

Stacy R. Finkbeiner, Binh-Minh Le, Lori R. Holtz, Gregory A. Storch, and David WangComments to Author 
Author affiliations: Washington University School of Medicine, St. Louis, Missouri, USA

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Appendix Figure

Validation of screening primers SF0073 and SF0076. Primers SF0073 and SF0076 were tested on stool filtrate made from the original AstV-MLB1-positive stool (lane 2) as well as a Human astrovirus 1-positive stool specimen (lane 3) using the QIAGEN One-Step reverse transcription-PCR (RT-PCR) Kit (QIAGEN, Valencia, CA, USA) as described in the text. The products were visualized by electrophoresis on a 1.2% agarose gel. The expected size of the RT-PCR product generated with these primers is approxima

Appendix Figure. Validation of screening primers SF0073 and SF0076. Primers SF0073 and SF0076 were tested on stool filtrate made from the original AstV-MLB1-positive stool (lane 2) as well as a Human astrovirus 1-positive stool specimen (lane 3) using the QIAGEN One-Step reverse transcription-PCR (RT-PCR) Kit (QIAGEN, Valencia, CA, USA) as described in the text. The products were visualized by electrophoresis on a 1.2% agarose gel. The expected size of the RT-PCR product generated with these primers is approximately 400 bp. Lane 1 shows the Invitrogen (Carlsbad, CA, USA) 100-bp DNA ladder for a size comparison.

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