Appendix Figure. Validation of screening primers SF0073 and SF0076. Primers SF0073 and SF0076 were tested on stool filtrate made from the original AstV-MLB1-positive stool (lane 2) as well as a Human astrovirus 1-positive stool specimen (lane 3) using the QIAGEN One-Step reverse transcription-PCR (RT-PCR) Kit (QIAGEN, Valencia, CA, USA) as described in the text. The products were visualized by electrophoresis on a 1.2% agarose gel. The expected size of the RT-PCR product generated with these primers is approximately 400 bp. Lane 1 shows the Invitrogen (Carlsbad, CA, USA) 100-bp DNA ladder for a size comparison.