Figure 1. A) Genetic relationships based on simple-sequence repeat (SSR) variation data among 183 Vibrio vulnificus isolates including 135 new environmental, 22 new clinical, and 26 previously studied isolates. B) A subtree enlargement of panel A displaying a set of 65 V. vulnificus biotype 3 isolates. Similar clinical and environmental isolates, showing an epidemiologic connection, are indicated by arrows. The genetic-distance matrix was generated based on 212 polymorphic points (the sum of alleles across 12 SSR loci). Genetic relationships are based on unweighted pair group method with arithmetic mean cluster analysis of SSR variation using MEGA4 software (11). Scale bar represents genetic distance.