Skip directly to local search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options
CDC Home

Volume 15, Number 8—August 2009

Research

Transgenic Mice Expressing Porcine Prion Protein Resistant to Classical Scrapie but Susceptible to Sheep Bovine Spongiform Encephalopathy and Atypical Scrapie

Juan-Carlos Espinosa1, María-Eugenia Herva1, Olivier Andréoletti, Danielle Padilla, Caroline Lacroux, Hervé Cassard, Isabelle Lantier, Joaquin Castilla, and Juan-María TorresComments to Author 
Author affiliations: Centro de Investigación en Sanidad Animal, Madrid, Spain (J.-C. Espinosa, M.-E. Herva, D. Padilla, J. Castilla, J.-M. Torres); École Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti, C. Lacroux, H. Cassard); Centre Institut National de la Recherche Agronomique de Tours, Nouzilly, France (I. Lantier)

Main Article

Figure 2

Brain atypical proteinase K–resistant prion protein (PrPres) of porcine PrP transgenic mice infected with cattle bovine spongiform encephalopathy (BSE) (lane 2) or sheep BSE agents (lane 4). Electrophoretic profiles and antibody labeling of PrPres detected with monoclonal antibodies Sha31 (A) or 12B2 (B). Profiles produced by cattle (lane 1) and sheep BSE (lane 3) before passage in the porcine mouse model are shown for comparison. MW, molecular mass in kilodaltons. C) Triangular plot of the glyc

Figure 2. Brain atypical proteinase K–resistant prion protein (PrPres) of porcine PrP transgenic mice infected with cattle bovine spongiform encephalopathy (BSE) (lane 2) or sheep BSE agents (lane 4). Electrophoretic profiles and antibody labeling of PrPres detected with monoclonal antibodies Sha31 (A) or 12B2 (B). Profiles produced by cattle (lane 1) and sheep BSE (lane 3) before passage in the porcine mouse model are shown for comparison. MW, molecular mass in kilodaltons. C) Triangular plot of the glycosyl fractions of PrPres after proteinase K digestion and Western blotting using the Sha31 antibody. Data shown are the means of 5 or more measurements obtained from density scans in 2 or more Western blots. To interpret the plot, read the values for the diglycosyl, monoglycosyl, and aglycosyl fractions along the bottom, right and left axes of the triangle, respectively. For each point, the sum of the 3 values is 100.

Main Article

1These authors contributed equally to this article.

Top of Page

 

Past Issues

Select a Past Issue:

Art in Science - Selections from Emerging Infectious Diseases
Now available for order



CDC 24/7 – Saving Lives, Protecting People, Saving Money. Learn More About How CDC Works For You…

USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO