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Volume 15, Number 9—September 2009

Research

Predicting Phenotype and Emerging Strains among Chlamydia trachomatis Infections

Deborah DeanComments to Author , William J. Bruno, Raymond Wan, João P. Gomes, Stéphanie Devignot, Tigist Mehari, Henry J.C. de Vries, Servaas A. Morré, Garry Myers, Timothy D. Read, and Brian G. Spratt
Author affiliations: Children’s Hospital Oakland Research Institute, Oakland, California, USA (D. Dean, R. Wan, T. Mehari); University of California, San Francisco, California, USA (D. Dean); University of California, Berkeley, California, USA (D. Dean); Los Alamos National Laboratories, Los Alamos, New Mexico, USA (W.J. Bruno); National Institute of Health, Lisbon, Portugal (J.P. Gomes); Institut de Médecine Tropicale du Service de Santé des Armées, Marseille, France (S. Devignot); University of Amsterdam, the Netherlands (H.J.C. de Vries); Vrije Universiteit Medical Center, Amsterdam (S.A. Morré); University of Maryland School of Medicine, Baltimore, Maryland, USA (G. Myers); Emory University, Atlanta, Georgia, USA (T.D. Read); Imperial College, London, UK (B.G. Spratt)

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Table 1

Primer pairs used for PCR of chlamydiaceae species and strains

Locus Region Primer name Sequence (5′ → 3′) Length of sequence, bp
glyA CT432 FglyA GAAGACTGTGGCGCTGTTTTATGG 522
RglyA
 CTTCCTGAGCGATCCCTTCTGAC
mdhC CT376 FmdhC GGAGATGTTTTTGGCCTTGATTGT 519
RmdhC
 CGATTACTGCACTACCACGACTCT
pdhA CT245 FpdhA CTACAGAAGCCCGAGTTTTT 549
RpdhA
 CTGTTTGTTGCATGTGGTGATAAG
yhbG CT653 FyhbG TCAAGTCAATGCAGGAGAAAT 504
RyhbG
 GATAGTGTTGACGTACCATAGGAT
pykF CT332 FpykF ATCTTATCGCTGCTTCGTT 525
RpykF
 cagcaataatagggagata
lysS CT781 FlysS GAAGGAATCGATAGAACGCATAAT 576
RlysS
 ATACGCCGCATAACAGGGAAAAAC
leuS CT209 FleuS TCCCTTGGTCGATCTCCTCAC 519
RleuS GGGCATCGCAAAAACGTAAATAGT

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