Volume 16, Number 12—December 2010
Letter
Molecular Detection of Bartonella alsatica in Rabbit Fleas, France
Tahar Kernif, Philippe Parola, Jean-Claude Ricci, Didier Raoult, and Jean-Marc Rolain
Table
Oligonucleotide primers and TaqMan* fluorescent probe sequences of hsp60 and gyrB genes used for reverse transcription PCRs of Bartonella alsatica†
| Gene |
Oligonucleotide |
Sequence (5′ → 3′) |
Length, bp |
Amplicon size, bp |
| hsp60 | B_alsa_hsp60_F | TGCTAACGCTATGGAAAAAGTTG | 23 | 108 |
| B_alsa_hsp60_R | CCACGATCAAACTGCATTCC | 20 | ||
| B_alsa_hsp60_P |
6FAM-TGTCGAAGAAGCAAAAACGGCTGAAACC-TAMRA |
28 |
||
| gyrB | B_alsa_gyrB_F | CGAAGCAAAACTTCTTATTAGTAAGGT | 27 | 126 |
| B_alsa_gyrB_R | GCAAGCTTTCCTGGCAGAG | 19 | ||
| B_alsa_gyrB_P | 6FAM-ATAGAGGCTGCTGCGGCGCG-TAMRA | 20 |
*Applied Biosystems, Courtaboeuf, France.
†hsp60, heat shock protein 60; gyrB, DNA gyrase subunit B.
