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Volume 16, Number 3—March 2010

Research

Use of Avian Bornavirus Isolates to Induce Proventricular Dilatation Disease in Conures

Patricia Gray, Sharman Hoppes, Paulette Suchodolski, Negin Mirhosseini, Susan Payne, Itamar Villanueva, H.L Shivaprasad, Kirsi S. Honkavuori, Thomas Briese, Sanjay M. Reddy, and Ian TizardComments to Author 
Author affiliations: Texas A&M University, College Station, Texas, USA (P. Gray, S. Hoppes, P. Suchodolski, N. Mirhosseini, S. Payne, I. Villanueva, S.M. Reddy, I. Tizard); California Animal Health and Food Safety Laboratory System, Fresno, California, USA (H.L. Shivaprasad); Columbia University, New York, New York, USA (K.S. Honkavuori, T. Briese)

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Figure 1

Western blot of infected duck embryonic fibroblasts (DEFs) showing avian bornavirus N-protein during culture. Lanes 1–4 are supernatant fluids. Lane I is from an African gray parrot (AG5). Lanes 2 and 3 are from a yellow-collared macaw (M24). Lane 4 is from uninfected DEFs. Lanes 5–8 are sonicated cell extracts. Lane 5 from AG5; 6 and 7 from M24; and Lane 8 from uninfected DEFs. Lane 9 is an infected brain control. The virus is strongly cell associated.

Figure 1. Western blot of infected duck embryonic fibroblasts (DEFs) showing avian bornavirus N-protein during culture. Lanes 1–4 are supernatant fluids. Lane I is from an African gray parrot (AG5). Lanes 2 and 3 are from a yellow-collared macaw (M24). Lane 4 is from uninfected DEFs. Lanes 5–8 are sonicated cell extracts. Lane 5 from AG5; 6 and 7 from M24; and Lane 8 from uninfected DEFs. Lane 9 is an infected brain control. The virus is strongly cell associated.

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