Volume 16, Number 3—March 2010
Research
Borrelia, Ehrlichia, and Rickettsia spp. in Ticks Removed from Persons, Texas, USA
Phillip C. Williamson
, Peggy M. Billingsley, Glenna J. Teltow, Janel P. Seals, Meredith A. Turnbough, and Samuel F. Atkinson
, Peggy M. Billingsley, Glenna J. Teltow, Janel P. Seals, Meredith A. Turnbough, and Samuel F. AtkinsonTable 1
Nucleotide sequence of primers used for PCR screening of tick specimens removed from humans, Texas, October 1, 2004, to September 30, 2008*
| Primer name |
Gene |
Primer sequence (5′ → 3′) |
Specificity |
Screen |
TM |
Reference |
|---|---|---|---|---|---|---|
| Tick DNA | ||||||
| 85F | 12S | TTAAGCTTTTCAGAGGAATTTGCTC | Unknown | Primary | 54.0 | This study |
| 225R | 12S | TTTWWGCTGCACCTTGACTTAA | Unknown | Primary | 52.7 | This study |
| Borrelia spp. | ||||||
| FlaLL | flaB | ACATATTCAGATGCAGACAGAGGT | Genus | Primary | 58.3 | (4) |
| FlaRL | flaB | GCAATCATAGCCATTGCAGATTGT | Genus | Primary | 58.9 | (4) |
| FlaLS | flaB | AACAGCTGAAGAGCTTGGAATG | Genus | Primary | 57.5 | (4) |
| FlaRS | flaB | CTTTGATCACTTATCATTCTAATAGC | Genus | Primary | 53.3 | (4) |
| BL-Fla 522F | flaB | GGTACATATTCAGATGCAGACAGAGGG | B. lonestari | Primary | 61.3 | This study |
| BL-Fla 1182R | flaB | GCACTTGATTTGCTTGTGCAATCATAGCC | B. lonestari | Primary | 64.0 | This study |
| BL-Fla 662F | flaB | CTGAAGAGCTTGGAATGCAACCTGC | B. lonestari | Primary | 62.8 | This study |
| BL-Fla 860R | flaB | GAGCTAATCCCACCTTGAGCTGG | B. lonestari | Primary | 61.2 | This study |
| BL-Fla 341F | flaB | AGCTGATGATGCTGCTGGTATGGG | Genus | Alternate | 63.2 | This study |
| BL-Fla 730R | flaB | GCTTGTGCTCCAGTTAGTGATGCTGG | Genus | Alternate | 64.1 | This study |
| BL-16S 227F | 16S | TCACACTGGAACTGAGATACGGTCC | Genus | Alternate | 62.1 | This study |
| BL-16S 920R | 16S | GAATTAAACCACATGCTCCACCGC | Genus | Alternate | 61.0 | This study |
| BL-HSP 71F | groEL | CTTATGTTGAAGGAATGCAATTTGA | B. lonestari | Alternate | 55.6 | This study |
| BL-HSP 271R |
groEL |
CAATATCTTCAGCAATAATTAGCAAAGGT |
B. lonestari |
Alternate |
58.2 |
This study |
| Rickettsia spp. | ||||||
| Rr.190 70P | rompA | ATGGCGAATATTTCTCCAAAA | Genus | Primary | 52.5 | (5) |
| Rr.190 602N | rompA | AGTGCAGCATTCGCTCCCCCT | Genus | Primary | 64.9 | (5) |
| BG1–21 | rompB | GGCAATTAATATCGCTGACGG | Genus | Alternate | 55.6 | (6) |
| BG2–20 | rompB | GCATCTGCACTAGCACTTTC | Genus | Alternate | 55.2 | (6) |
| RrCS 372 | gltA | TTTGTAGCTCTTCTCATCCTATGGC | Genus | Alternate | 59.0 | (7) |
| RrCS 989 | gltA | CCCAAGTTCCTTTAATACTTCTTTGC | Genus | Alternate | 57.5 | (7) |
| Primer 1 | 17kDa | GCTCTTGCAACTTCTATGTT | Genus | Alternate | 52.3 | (8) |
| Primer 2 |
17kDa |
CATTGTTCGTCAGGTTGGCG |
Genus |
Alternate |
57.9 |
(8) |
| Ehrlichia spp. | ||||||
| Ehr DSB 330F | dsb | GATGATGTCTGAAGATATGAAACAAAT | Genus | Primary | 55.5 | (9) |
| Ehr DSB 728R | dsb | CTGCTCGTCTATTTTACTTCTTAAAGT | Genus | Primary | 56.6 | (9) |
| ECC-F | 16S | AGAACGAACGCTGGCGGCAAGCC | Genus | Alternate | 68.1 | (10) |
| ECB-R | 16S | CGTATTACCGCGGCTGCTGGCA | Genus | Alternate | 65.6 | (10) |
| ECAN-F | 16S | ATTTATAGCCTCTGGCTATAGGA | E. canis | Alternate | 54.9 | (11) |
| HE1-F | 16S | CAATTGCTTATAACCTTTTGGTTATAAAT | E. chaffeensis | Alternate | 55.6 | (12) |
| EE72-F | 16S | AATTCCTAAATAGTCTCTGACTATT | E. ewingii | Alternate | 52.6 | (11) |
| HE3-R | 16S | TATAGGTACCGTCATTATCTTCCCTAT | Genus | Alternate | 57.6 | (13) |
*TM, melting temperature, °C.
