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Volume 16, Number 3—March 2010

Research

Borrelia, Ehrlichia, and Rickettsia spp. in Ticks Removed from Persons, Texas, USA

Phillip C. WilliamsonComments to Author , Peggy M. Billingsley, Glenna J. Teltow, Janel P. Seals, Meredith A. Turnbough, and Samuel F. Atkinson
Author affiliations: University of North Texas Health Science Center, Fort Worth, Texas, USA (P.C. Williamson, P.M. Billingsley, J.P. Seals); Texas Department of State Health Services, Temple, Texas, USA (G.J. Teltow); University of North Texas, Denton, Texas, USA (M.A. Turnbough, S.F. Atkinson)

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Table 1

Nucleotide sequence of primers used for PCR screening of tick specimens removed from humans, Texas, October 1, 2004, to September 30, 2008*

Primer name
Gene
Primer sequence (5′ → 3′)
Specificity
Screen
TM
Reference
Tick DNA
85F 12S TTAAGCTTTTCAGAGGAATTTGCTC Unknown Primary 54.0 This study
225R 12S TTTWWGCTGCACCTTGACTTAA Unknown Primary 52.7 This study
Borrelia spp.
FlaLL flaB ACATATTCAGATGCAGACAGAGGT Genus Primary 58.3 (4)
FlaRL flaB GCAATCATAGCCATTGCAGATTGT Genus Primary 58.9 (4)
FlaLS flaB AACAGCTGAAGAGCTTGGAATG Genus Primary 57.5 (4)
FlaRS flaB CTTTGATCACTTATCATTCTAATAGC Genus Primary 53.3 (4)
BL-Fla 522F flaB GGTACATATTCAGATGCAGACAGAGGG B. lonestari Primary 61.3 This study
BL-Fla 1182R flaB GCACTTGATTTGCTTGTGCAATCATAGCC B. lonestari Primary 64.0 This study
BL-Fla 662F flaB CTGAAGAGCTTGGAATGCAACCTGC B. lonestari Primary 62.8 This study
BL-Fla 860R flaB GAGCTAATCCCACCTTGAGCTGG B. lonestari Primary 61.2 This study
BL-Fla 341F flaB AGCTGATGATGCTGCTGGTATGGG Genus Alternate 63.2 This study
BL-Fla 730R flaB GCTTGTGCTCCAGTTAGTGATGCTGG Genus Alternate 64.1 This study
BL-16S 227F 16S TCACACTGGAACTGAGATACGGTCC Genus Alternate 62.1 This study
BL-16S 920R 16S GAATTAAACCACATGCTCCACCGC Genus Alternate 61.0 This study
BL-HSP 71F groEL CTTATGTTGAAGGAATGCAATTTGA B. lonestari Alternate 55.6 This study
BL-HSP 271R
groEL
CAATATCTTCAGCAATAATTAGCAAAGGT
B. lonestari
Alternate
58.2
This study
Rickettsia spp.
Rr.190 70P rompA ATGGCGAATATTTCTCCAAAA Genus Primary 52.5 (5)
Rr.190 602N rompA AGTGCAGCATTCGCTCCCCCT Genus Primary 64.9 (5)
BG1–21 rompB GGCAATTAATATCGCTGACGG Genus Alternate 55.6 (6)
BG2–20 rompB GCATCTGCACTAGCACTTTC Genus Alternate 55.2 (6)
RrCS 372 gltA TTTGTAGCTCTTCTCATCCTATGGC Genus Alternate 59.0 (7)
RrCS 989 gltA CCCAAGTTCCTTTAATACTTCTTTGC Genus Alternate 57.5 (7)
Primer 1 17kDa GCTCTTGCAACTTCTATGTT Genus Alternate 52.3 (8)
Primer 2
17kDa
CATTGTTCGTCAGGTTGGCG
Genus
Alternate
57.9
(8)
Ehrlichia spp.
Ehr DSB 330F dsb GATGATGTCTGAAGATATGAAACAAAT Genus Primary 55.5 (9)
Ehr DSB 728R dsb CTGCTCGTCTATTTTACTTCTTAAAGT Genus Primary 56.6 (9)
ECC-F 16S AGAACGAACGCTGGCGGCAAGCC Genus Alternate 68.1 (10)
ECB-R 16S CGTATTACCGCGGCTGCTGGCA Genus Alternate 65.6 (10)
ECAN-F 16S ATTTATAGCCTCTGGCTATAGGA E. canis Alternate 54.9 (11)
HE1-F 16S CAATTGCTTATAACCTTTTGGTTATAAAT E. chaffeensis Alternate 55.6 (12)
EE72-F 16S AATTCCTAAATAGTCTCTGACTATT E. ewingii Alternate 52.6 (11)
HE3-R 16S TATAGGTACCGTCATTATCTTCCCTAT Genus Alternate 57.6 (13)

*TM, melting temperature, °C.

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