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Volume 16, Number 3—March 2010

Dispatch

Experimental Infection of Squirrel Monkeys with Nipah Virus

Philippe Marianneau1, Vanessa Guillaume1, K. Thong Wong, Munisamy Badmanathan, Ren Yih Looi, Séverine Murri, Philippe Loth, Noël Tordo, T. Fabian Wild, Branka HorvatComments to Author , and Hugues Contamin
Author affiliations: Institut Pasteur, Lyon, France (P. Marianneau, S. Murri, P. Loth, N. Tordo, H. Contamin); Institut national de santé et de la recherché médicale, Lyon (V. Guillaume, T.F. Wild, B. Horvat); University of Malaya, Kuala Lumpur, Malaysia (K.T. Wong, M. Badmanathan, R.Y. Looi)

Main Article

Table 2

Detection of anti–Nipah virus antibodies in 12 squirrel monkeys by ELISA and seroneutralization assay*

Monkey Mode of infection† Serology‡ Day postinfection
2 3 or 4 8 or 9 12 17 30 37 52 or 56
IV 103 IgM Neg
IV 103 IgG Neg

IV 103
Neutralization

Neg






IV 103 IgM Neg Neg 0.396
IV 103 IgG Neg Neg Neg

IV 103
Neutralization
Neg

Neg
Neg




IV 103 IgM Neg 0.664
IV 103 IgG Neg Neg

IV 103
Neutralization
Neg

Neg





IV 107 IgM Neg
IV 107 IgG Neg

IV 107
Neutralization

Neg






IV 107 IgM Neg 1.343
IV 107 IgG Neg 0.562

IV 107
Neutralization
Neg

40





F IV 107 IgM Neg 1.550 0.374 0.175
IV 107 IgG Neg 0.369 2.867 3.023

IV 107
Neutralization
Neg

80


>1,280

>1,280
IN 103 IgM Neg Neg
IN 103 IgG Neg Neg

IN 103
Neutralization
Neg
Neg






H IN 103 IgM Neg Neg Neg
IN 103 IgG Neg Neg Neg

IN 103
Neutralization


Neg


Neg

Neg
I IN 103 IgM Neg Neg Neg Neg
IN 103 IgG Neg Neg Neg Neg

IN 103
Neutralization


Neg


Neg
Neg
Neg
IN 107 IgM Neg
IN 107 IgG Neg

IN 107
Neutralization

Neg






K IN 107 IgM Neg 0.286
IN 107 IgG Neg 1.757

IN 107
Neutralization
Neg



80



L IN 107 IgM 0.375 0.248 Neg
IN 107 IgG Neg 2.078 2.308
IN 107 Neutralization Neg 320 320

*IV, intravenous; Ig, immunoglobulin; IN, intranasal; dpi, days postinfection.
†Mode of infection and delivered viral dose (in PFU).
‡IgG and IgM antibodies were determined by ELISA and results are presented as absorbance readings. Neutralization titers are expressed as reciprocal values of 2-fold serum dilutions required to completely inhibit cytopathic effect of 25 PFU of Nipah virus on Vero cells.
§Early systemic euthanasia: 3 dpi (A and D) or 4 dpi (G and J).
¶Death caused by Nipah virus infection, B: 12 dpi, C: 21 dpi, and E: 8 dpi.

Main Article

1These authors contributed equally to this article.

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