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Volume 16, Number 4—April 2010

Research

Escherichia albertii in Wild and Domestic Birds

J. Lindsay OaksComments to Author , Thomas E. Besser, Seth T. Walk, David M. Gordon, Kimberlee B. Beckmen, Kathy A. Burek, Gary J. Haldorson, Dan S. Bradway, Lindsey Ouellette, Fred R. Rurangirwa, Margaret A. Davis, Greg Dobbin, and Thomas S. Whittam1
Author affiliations: Washington State University, Pullman, Washington, USA (J.L. Oaks, T.E. Besser, G.J. Haldorson, D.S. Bradway, F.R. Rurangirwa, M.A. Davis); University of Michigan Health System, Ann Arbor, Michigan, USA (S.T. Walk); The Australian National University, Canberra, Australian Capital Territory, Australia (D.M. Gordon); Alaska Department of Fish and Game, Fairbanks, Alaska, USA (K.B. Beckmen); Alaska Veterinary Pathology Services, Eagle River, Alaska, USA (K.A. Burek); Michigan State University, East Lansing, Michigan, USA (L. Ouellette, T.S. Whittam); University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada (G. Dobbin); 1Deceased.

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Table 3

Primer sets used for amplification and sequencing of eae gene of Escherichia albertii in specific isolates

Isolate PCR amplification Sequencing
1297–05–19 Intimin γ F, intimin γ R Intimin γ F, intimin γ R
7991–07 EaeA-F, EaeA-R outer EaeA-F, EaeA-R inner
12055–07 Intimin γ F, intimin γ R Intimin γ F, intimin γ R
5419–05-R Intimin γ F, intimin γ R Intimin γ F, intimin γ R
EC370–98 Intimin γ F, intimin γ R Intimin γ F, intimin γ R
EC746–99 Intimin γ F, intimin γ R Intimin γ F, intimin γ R
B101 Intimin γ F, intimin γ R Intimin γ F, intimin γ R
B156 EaeA-F, Intimin-R2 EaeA-F, intimin-R2, intimin B156 int R
B198 EaeA-F, intimin-R2 EaeA-F, intimin-R2, intimin B156 int R
B992 EaeA F06s, EscD-R1 EaeA F06s, EscD-R1, EaeA-F, intimin B101 int R
B1086 EaeA-F, intimin γ R EaeA-F, intimin γ R, EaeA-R outer
B1068 EaeA F06s, EscD-R1 EaeA F06s, EscD-R1, EaeA-F
B1074 EaeA F06s, EscD-R1 EaeA F06s, EscD-R1, EaeA-F

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