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Volume 16, Number 6—June 2010

Dispatch

Pneumovirus in Dogs with Acute Respiratory Disease

Randall W. Renshaw, Nancy C. Zylich, Melissa A. Laverack, Amy L. Glaser, and Edward J. DuboviComments to Author 
Author affiliations: Cornell University, Ithaca, New York, USA

Main Article

Table

Partial gene locations of virus isolated from dogs with respiratory disease and relatedness to other pneumoviruses*

Region sequenced† PCR primers (5′ → 3′) and aa target sequences in pneumoviruses % Identity to MPV, nt, aa† % Identity to HRSV, nt, aa‡ % Identity to HMPV, nt, aa§
N gene,¶ nt 852–1182 (331 bp)
P1R: ggaactcgggggcgaayttytccat
target: MEKFAPEFH
N276F: tccgtgcaggccgaratggarcarg
target: SVQAEMEQV
96.4, 96.3
68.5, 70.1
56.5, 54.1
L gene, no. 1,¶ nt 1143–1452 (310 bp)
L428F: ccggatcttcggccayccnatggt
target: RIFGHPMV
L538R: ttcttaggaggggagatggcyttrtcrtt
target: NDKAISPPKN
95.8, 97.1
62.1, 55.3
58.9, 47.6
L gene, no. 2,¶ nt 1962–2511 (550 bp) L698F: catcaccgacctgtccaagttyaaycargc
target: ITDLSKFNQA
L894R: ttgaagtcgtccaggatggtrttdatcca
target: WINTILDDFK 96.7, 99.5 68.4, 71.6 66.4, 68.3

*MPV, murine pneumovirus; nt, nucleotide; aa, amino acid; HRSV, human respiratory syncytial virus; HMPV, human metapneumovirus; N, nucleocapsid; R, reverse primer; F, forward primer L, polymerase.
†Based on comparison with MPV strain J3666, GenBank accession no. NC_006579.
‡Based on comparison with HRSV, GenBank accession no. NC_001803.
§Based on comparison with HMPV, GenBank accession no. NC_004148.
¶The N gene fragment is entered under GenBank accession no. GU247050; L gene fragments nos. 1 and 2 are entered under GenBank accession no. GU247051.

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