Volume 16, Number 6—June 2010
Pneumovirus in Dogs with Acute Respiratory Disease
|Region sequenced†||PCR primers (5′ → 3′) and aa target sequences in pneumoviruses||% Identity to MPV, nt, aa†||% Identity to HRSV, nt, aa‡||% Identity to HMPV, nt, aa§|
|N gene,¶ nt 852–1182 (331 bp)
|L gene, no. 1,¶ nt 1143–1452 (310 bp)
|L gene, no. 2,¶ nt 1962–2511 (550 bp)||L698F: catcaccgacctgtccaagttyaaycargc target: ITDLSKFNQA L894R: ttgaagtcgtccaggatggtrttdatcca target: WINTILDDFK||96.7, 99.5||68.4, 71.6||66.4, 68.3|
*MPV, murine pneumovirus; nt, nucleotide; aa, amino acid; HRSV, human respiratory syncytial virus; HMPV, human metapneumovirus; N, nucleocapsid; R, reverse primer; F, forward primer L, polymerase.
†Based on comparison with MPV strain J3666, GenBank accession no. NC_006579.
‡Based on comparison with HRSV, GenBank accession no. NC_001803.
§Based on comparison with HMPV, GenBank accession no. NC_004148.
¶The N gene fragment is entered under GenBank accession no. GU247050; L gene fragments nos. 1 and 2 are entered under GenBank accession no. GU247051.
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