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Volume 17, Number 12—December 2011
Research

Lineage and Virulence of Streptococcus suis Serotype 2 Isolates from North America

Nahuel Fittipaldi, Jiangu Xu, Sonia Lacouture, Prasit Tharavichitkul, Makoto Osaki, Tsutomu Sekizaki, Daisuke Takamatsu, and Marcelo GottschalkComments to Author 
Author affiliations: Université de Montréal, St-Hyacinthe, Quebec, Canada (N. Fittipaldi, S. Lacouture, M. Gottschalk); National Institute for Communicable Disease Control and Prevention, Beijing, People’s Republic of China (J. Xu); Chiang Mai University, Chiang Mai, Thailand (P. Tharavichitkul); Agriculture, Forestry and Fisheries Research Council, Tokyo, Japan (M. Osaki); The University of Tokyo, Tokyo (T. Sekizaki); National Institute of Animal Health, Tsukuba, Japan (D. Takamatsu)

Main Article

Table 2

Association of Streptococcus suis serotype 2 STs and commonly used virulence markers in isolates from North America†

ST No. strains Presence of factor-encoding gene
 Phenotype
sly mrp variant‡
 epf
mrp mrps mrp* mrp** mrp*** ND Hemolysis§ MRP¶ EF
1 11 11 9 0 0 0 0 2 11 11 9 11
25 36 0 0 1 1 8 23 3 0 0 0 0
28 49 0 42 6 1 0 0 0 3 0 49 0

†ST, sequence type; SLY, suilysin; MRP, muraminidase-released protein; ND, no amplification of the mrp gene was detected by PCR under the conditions used; EF, extracellular factor.
‡Variants of the mrp gene are those described by Silva et al. (20).
§Hemolysis of horse erythrocytes by the strains was considered to be an indication of the expression of SLY.
¶Molecular mass MRP variants identified by Western blotting were in agreement with those expected on the basis of the mrp gene variant identified by PCR.

Main Article

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Page created: November 30, 2011
Page updated: November 30, 2011
Page reviewed: November 30, 2011
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