Emerging Infectious Disease ISSN: 1080-6059

Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Steve Ahuka-Mundeke, Ahidjo Ayouba, Placide Mbala-Kingebeni, Florian Liegeois, Amandine Esteban, Octavie Lunguya-Metila, Didace Demba, Guy Bilulu, Valentin Mbenzo-Abokome, Bila-Isia Inogwabini, Jean-Jacques Muyembe-Tamfum, Eric Delaporte, and Martine Peeters

Author affiliations: University of Montpellier, Montpellier, France (S. Ahuka-Mundeke, A. Ayouba, F. Liegeois, A. Esteban, E. Delaporte, M. Peeters); Institut National de Recherche Biomédicales, Kinshasa, Democratic Republic of Congo (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Cliniques Universitaires de Kinshasa, Kinshasa (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Zone de Santé de Kole, Sankuru, Kasai Oriental, Democratic Republic of Congo (D. Demba, G. Bilulu); World Wildlife Fund For Nature, Kinshasa (V. Mbenzo-Abokome, B-I. Inogwabini)

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Figure 2

Phylogenetic relationships of the newly derived simian immunodeficiency virus (SIV) sequences in pol to representatives of the other SIV lineages. Newly identified strains in this study are in red and reference strains are in black. Unrooted trees were inferred from 350-bp nucleotides. Analyses were performed by using discrete gamma distribution and a generalized time reversible model. The starting tree was obtained by using phyML (27). One hundred bootstrap replications were performed to assess

Figure 2. Phylogenetic relationships of the newly derived simian immunodeficiency virus (SIV) sequences in pol to representatives of the other SIV lineages. Newly identified strains in this study are in red and reference strains are in black. Unrooted trees were inferred from 350-bp nucleotides. Analyses were performed by using discrete gamma distribution and a generalized time reversible model. The starting tree was obtained by using phyML (27). One hundred bootstrap replications were performed to assess confidence in topology. Numbers at nodes are from 100 bootstrap replicates; only those >90% are shown with an asterisk. Scale bar represents nucleotide replacements per site.

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