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Volume 17, Number 12—December 2011

Research

Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Steve Ahuka-Mundeke, Ahidjo Ayouba, Placide Mbala-Kingebeni, Florian Liegeois, Amandine Esteban, Octavie Lunguya-Metila, Didace Demba, Guy Bilulu, Valentin Mbenzo-Abokome, Bila-Isia Inogwabini, Jean-Jacques Muyembe-Tamfum, Eric Delaporte, and Martine PeetersComments to Author 
Author affiliations: University of Montpellier, Montpellier, France (S. Ahuka-Mundeke, A. Ayouba, F. Liegeois, A. Esteban, E. Delaporte, M. Peeters); Institut National de Recherche Biomédicales, Kinshasa, Democratic Republic of Congo (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Cliniques Universitaires de Kinshasa, Kinshasa (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Zone de Santé de Kole, Sankuru, Kasai Oriental, Democratic Republic of Congo (D. Demba, G. Bilulu); World Wildlife Fund For Nature, Kinshasa (V. Mbenzo-Abokome, B-I. Inogwabini)

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Table 4

Sensitivity and specificity of SIV/HIV peptides used in the xMAP assay to detect SIV infection in human and nonhuman primate samples*

Species (common name) Peptide Homologous detection
Homologous and heterologous detection
SIV+
SIV–
SIV+
SIV–
gp41 V3 gp41 + V3 gp41 V3 gp41 + V3 gp41 V3 gp41 V3
Pan troglodytes troglodytes (west central chimpanzee) SIVcpzPtt 1/2 1/2 1/2 0/1 0/1 0/1 3/49 4/49 0/93 0/93
Pan troglodytes schweinfurthii (eastern chimpanzee) SIVcpzPts 1/1 1/1 −/− −/− −/− −/− 8/49 13/49 0/93 0/93
Gorilla gorilla gorilla (western lowland gorilla) SIVgor −/− −/− −/− −/− −/− −/− 1/49 0/49 0/93 0/93
Cercopithecus nictitans (greater spot-nosed monkey) SIVgsn 5/6 6/6 6/6 0/45 0/45 0/45 14/49 15/49 0/93 0/93
Cercopithecus cephus (mustached monkey) SIVmus 7/7 7/7 7/7 0/30 0/30 0/30 23/49 15/49 0/93 0/93
Cercopithecus mona (Mona monkey) SIVmon −/− −/− −/− 0/1 0/1 0/1 12/49 15/49 0/93 0/93
Miopithecus ogouensis (northern talapoin) SIVtal 1/1 −/− −/− 0/6 0/6 0/6 6/49 −/− 0/93 0/93
Cercopithecus neglectus (De Brazza monkey) SIVdeb 7/7 6/7 7/7 0/1 0/1 0/1 19/49 6/49 0/93 0/93
Cercopithecus albogularis (Sykes’ monkey) SIVsyk −/− −/− −/− −/− −/− −/− 11/49 1/49 0/93 0/93
Cercocebus atys (sooty mangabey) SIVsmm −/− −/− −/− −/− −/− −/− 30/49 1/49 0/93 0/93
Cercocebus torquatus (red-capped mangabey) SIVrcm 4/4 4/4 4/4 −/− −/− −/− 7/49 4/49 0/93 0/93
Chlorocebus tantalus (African green monkey) SIVagm 1/1 1/1 1/1 −/− −/− −/− 5/49 3/49 0/93 2/93
Mandrillus sphinx (mandrill) SIVmnd-2 7/10 10/10 10/10 0/2 0/2 0/2 13/49 11/49 0/93 0/93
Cercopithecus lhoesti (L'Hoest's monkey) SIVlho −/− −/− −/− −/− −/− −/− 6/49 6/49 0/93 0/93
Procolobus badius (western red colobus) SIVwrc −/− −/− −/− −/− −/− −/− 1/49 6/49 0/93 0/93
Colobus guereza (mantled guereza) SIVcol −/− 9/10 9/10 −/− 0/7 0/7 −/− 9/49 0/93 0/93
Homo sapiens (human) HIV-1M −/− −/− −/− −/− −/− −/− 4/49 1/49 0/93 0/93
HIV-1-O −/− −/− −/− −/− −/− −/− 1/49 0/49 0/93 0/93
HIV-1N −/− −/− −/− −/− −/− −/− 9/49 1/49 0/93 0/93
Total 34/39 46/49 47/49 0/87 0/87 0/87 49/49 47/49 0/93 2//93
Sensitivity 87.2% 93.9% 95.9% NA NA NA 100% 95.5% NA NA
Specificity NA NA NA 100% 100% 100% NA NA 100% 97.9%

*SIV, simian immunodeficiency virus; −/−, not calculated because homologous serum and/or peptide not available; NA, not applicable.

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