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Volume 17, Number 12—December 2011

Research

Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Steve Ahuka-Mundeke, Ahidjo Ayouba, Placide Mbala-Kingebeni, Florian Liegeois, Amandine Esteban, Octavie Lunguya-Metila, Didace Demba, Guy Bilulu, Valentin Mbenzo-Abokome, Bila-Isia Inogwabini, Jean-Jacques Muyembe-Tamfum, Eric Delaporte, and Martine PeetersComments to Author 
Author affiliations: University of Montpellier, Montpellier, France (S. Ahuka-Mundeke, A. Ayouba, F. Liegeois, A. Esteban, E. Delaporte, M. Peeters); Institut National de Recherche Biomédicales, Kinshasa, Democratic Republic of Congo (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Cliniques Universitaires de Kinshasa, Kinshasa (S. Ahuka-Mundeke, P. Mbala-Kingebeni, O. Lunguya-Metila, J.-J. Muyembe-Tamfum); Zone de Santé de Kole, Sankuru, Kasai Oriental, Democratic Republic of Congo (D. Demba, G. Bilulu); World Wildlife Fund For Nature, Kinshasa (V. Mbenzo-Abokome, B-I. Inogwabini)

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Table 5

Number and percentage of SIV antibody–positive samples per species and number of samples confirmed by PCR and sequence analysis per species and per site*

Species (common name) Antibody detection, no. (%)
PCR, no. pos/no. tested
Kole

Malebo

Monkoto†

Total
Tested Pos Ind Tested Pos Ind Tested Pos Tested Pos Ind
Allopithecus negroviridis (Allen's swamp monkey) 1 0 0 NA NA NA 2 0 3 0 0 Not done
Colobus angolensis (Angolan colobus) 21 1 (5.0) 0 NA NA NA 4 1 (25.0) 25 1 (4.0) 0 0/1
Cercopithecus ascanius (red-tailed monkey) 94 23 (24.4) 4 (4.2) 40 12 (30.0) 3 (7.5) 13 3 (23.0) 147 37 (25.0) 7 (4.7) 4/37
Cercopithecus neglectus (De Brazza monkey) 7 2 (28.0) 0 2 0 0 1 0 10 2 (20) 0 1/2
Cercopithecus wolfi (Wolf's monkey) 27 5 (19.0) 3 (11.0) 1 0 0 5 3 (60.0) 33 4 (12.1) 3 (9.1) 1/4
Lophocebus atterimus (black mangabey) 30 2 (6.0) 0 NA NA NA 3 0 33 1 (3.0) 0 0/1
Piliocolobus tholloni (Tshuapa red colobus) 78 19 (24.0) 0 NA NA NA 1 0 79 19 (24.1) 0 2/19
Total 258 52 (20.0) 7 (3.0) 43 12 (27.0) 3 (7.0) 29 7 (21.0) 330 64 (19.0) 10 (3.0) 8/64

*SIV, simian immunodeficiency virus; pos, positive; ind, indeterminate; NA, not applicable (samples not available). Samples were scored as SIV positive when both assays (xMAP and HIV INNO-LIA) were clearly positive or positive in 1 assay (MFI/cutoff >2 or reactivity with at least 1 HIV antigen with a band intensity equal to or greater than the assay cutoff). Samples were scored indeterminate (ind) when reactive in HIV INNO-LIA with at least one HIV antigen with a band intensity equal to or greater than the assay cut-off and weakly positive (MFI/cut-off ratio between 1 and 2) in the other assay or when both assays were indeterminate; samples were considered as negative when both test results were negative.
†No samples from Monkoto were indeterminate.

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