Volume 17, Number 12—December 2011
Dispatch
Japanese Encephalitis Virus Genotype Replacement, Taiwan, 2009–2010
Yi-Ying Chen1, Yi-Chin Fan1, Wu-Chun Tu1, Rey-Yi Chang, Chen-Chang Shih, In-Houng Lu, Maw-Shien Chien, Wei-Cheng Lee, Ter-Hsin Chen, Gwong-Jen Chang, and Shyan-Song Chiou
Table
The 3 primer pairs used in reverse transcription PCR to differentiate genotypes I and III Japanese encephalitis virus, Taiwan, 2009–2010*
| Specificity/ name | Sequence, 5′ → 3′ | Location† |
|---|---|---|
| All JEV | ||
| JE1F | TGTGTGAACTTCTTGGCTTAGTAT | 12–35 |
| JE1R | CARCATCTGTTYTCWCCTTTTGA | 559–581 |
| GI JEV only | ||
| GIJEVF | CAGTCGCGAGTTTAAACGAC | 2000–2019 |
| GIJEVR | CATTCAGTTCGTCCCGCACA | 2688–2707 |
| GIII JEV only | ||
| GIIIJEVF | GGATGCTTGGCAGTAACAAC | 904–923 |
| GIIIJEVR | AAGTCCACATCCGTTGCC | 1281–1298 |
*JEV, Japanese encephalitis virus; GI, genotype I; GIII, genotype III.
†According to JEV strain T1P1 complete genome (GenBank accession no. AF254453).
1These authors contributed equally to this article.
