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Volume 17, Number 4—April 2011

Dispatch

Molecular Discrimination of Sheep Bovine Spongiform Encephalopathy from Scrapie

Laura Pirisinu, Sergio Migliore, Michele Angelo Di Bari, Elena Esposito, Thierry Baron, Claudia D’Agostino, Luigi De Grossi, Gabriele Vaccari, Umberto Agrimi, and Romolo NonnoComments to Author 
Author affiliations: Author affiliations: Istituto Superiore di Sanità, Rome, Italy (L. Pirisinu, S. Migliore, M.A. Di Bari, E. Esposito, C. D’Agostino, G. Vaccari, U. Agrimi, R. Nonno); Agence Nationale de Sécurité Sanitaire, Lyon, France (T. Baron); Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Rome (L. De Grossi)

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Table 2

Transmissible spongiform encephalopathy samples analyzed by discriminatory Western blot*

Source Identification no. PrP genotype† Blot
lane
Natural isolates
Scrapie ES16/10/10 ARQ/ARQ Sc1
ES16/10/11 ARQ/ARQ Sc2
ES16/10/12 ARQ/ARQ Sc3
ES12/10/1 ARQ/ARQ
ES12/10/2 ARQ/ARQ
ES12/10/3 ARQ/ARQ Sc4
CH1641-like 99–454 VRQ/VRQ Ch2
99–321 VRQ/VRQ Ch4
TR316211 ARQ/ARQ Ch3
Goat BSE
CH636

Bs6
Experimental samples
CH1641 241/74 AxQ/AxQ Ch1
Sheep BSE 301/16‡ ARQ/ARQ Bs1
301/44‡ ARQ/ARQ
302/87§ ARQ/ARQ Bs3
302/130§ ARQ/ARQ Bs4
302/64§ ARQ/AHQ Bs5
302/90§ ARQ/ARQ Bs2

*Blot lanes shown in Figure 1. PrP, prion protein; BSE, bovine spongiform encephalopathy.
†Amino acids at codons 136, 154 and 171.
‡Intracerebral transmission.
§Oral transmission.

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