Emerging Infectious Disease ISSN: 1080-6059

Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease

Silvio Notari¹, Liuting Qing¹, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi Gambetti

Author affiliations: Case Western Reserve University, Cleveland, Ohio, USA (S. Notari, L. Qing, A. Dagdanova, K. Hatcher, S.G. Chen, Q. Kong, P. Gambetti); Istituto Superiore di Sanità, Rome, Italy (M. Pocchiari, M. Puopolo); Ferring Pharmaceuticals, Hvidore, Denmark (A. Dogterom); Instituto Massone, Buenos Aires, Argentina (J.F. Groisman); BL Consult ApS, Copenhagen, Denmark (I.B. Lumholtz); The Scripps Research Institute, Jupiter, Florida, USA (C. Lasmezas)

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Figure 1

Figure 1. Dose-incubation period curve of brain microsomal fraction from sporadic Creutzfeldt-Jakob disease MM1 (patient 1) intracerebrally injected into Tg40 mice. Each solid circle represents the incubation time for single animal (x-axis) at different brain tissue equivalent dilution. Animals with the same incubation times have overlapping solid circles. Right y-axis is the amount of infectivity present in the inoculum at each brain tissue equivalent dilution (left y-axis). The experimental points were fitted by linear regression curve and 50% infectious dose (ID₅₀) calculated by using probit-nonlinear regression analysis. Dashed lines indicate 95% confidence interval.

Main Article

¹These authors contributed equally to this article.

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