Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease
Silvio Notari1, Liuting Qing1, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi Gambetti
Author affiliations: Case Western Reserve University, Cleveland, Ohio, USA (S. Notari, L. Qing, A. Dagdanova, K. Hatcher, S.G. Chen, Q. Kong, P. Gambetti); Istituto Superiore di Sanità, Rome, Italy (M. Pocchiari, M. Puopolo); Ferring Pharmaceuticals, Hvidore, Denmark (A. Dogterom); Instituto Massone, Buenos Aires, Argentina (J.F. Groisman); BL Consult ApS, Copenhagen, Denmark (I.B. Lumholtz); The Scripps Research Institute, Jupiter, Florida, USA (C. Lasmezas)
Figure 1. Dose-incubation period curve of brain microsomal fraction from sporadic Creutzfeldt-Jakob disease MM1 (patient 1) intracerebrally injected into Tg40 mice. Each solid circle represents the incubation time for single animal (x-axis) at different brain tissue equivalent dilution. Animals with the same incubation times have overlapping solid circles. Right y-axis is the amount of infectivity present in the inoculum at each brain tissue equivalent dilution (left y-axis). The experimental points were fitted by linear regression curve and 50% infectious dose (ID50) calculated by using probit-nonlinear regression analysis. Dashed lines indicate 95% confidence interval.
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