Volume 18, Number 1—January 2012
Research
Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease
Silvio Notari1, Liuting Qing1, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi Gambetti
Table 3
Determination of infectivity loss of MF from sCJDMM1 patient 3 spiked in normal urine, during concentration and dialysis procedures*
| Inoculum | Brain† dilution | Incubation time, d, mean ± SEM | Distribution | Prion titer, ID50/g tissue† |
|---|---|---|---|---|
| MF in normal urine, then concentrated 100× and dialyzed | 10−2 | 265 ± 12‡ | 8/9 | 8.2 × 106 |
| MF in 100× concentrated and dialyzed normal human urine | 10−2 | 268 ± 10‡ | 8/8 | 6.9 × 106 |
*MF, microsomal fraction; sCJD, sporadic Creutzfeldt-Jakob disease; ID50, 50% infectious dose.
†Brain tissue equivalent.
‡p = 0.42 by t-test.
1These authors contributed equally to this article.
